Analytical grade methanol

Manufactured by Sangon

Sourced in China

Analytical-grade methanol is a high-purity solvent used in various laboratory applications. It serves as a critical component in analytical techniques, providing a reliable and consistent medium for chemical reactions, sample preparation, and instrumental analysis.

Automatically generated - may contain errors

Lab products found in correlation

Phosphoric acid, by Sangon (4 mentions) E2695, by Waters Corporation (3 mentions) Nahco3, by Sangon (2 mentions) Phosphoric acid, by Waters Corporation (2 mentions) Methanol, by Waters Corporation (2 mentions) Waters e2695, by Waters Corporation (1 mentions)

Close spelling variants of this product

Methanol (24 citations)

4 protocols using analytical grade methanol

Quantification of Aloe-Emodi Bioactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard aloe-emodi, rhein, emodin, chrysophanol, and physcion were obtained from Shanghai R&D Center for Standardization of Traditional Chinese Medicines. High-performance liquid chromatography (HPLC)-ultrapure water, analytical-grade methanol, NaHCO₃, and phosphoric acid were purchased from Sangon Biotech, Ltd. (Shanghai, China).
According to the Chinese Pharmacopoeia (Edition 2015) [18 ], the dried root of each treatment specimen (three replications) was pulverized and sieved through a 300 μm mesh. A total of 1.5 g of powder of each sample was precisely weighed, added to 10 mL 0.1% NaHCO₃ aqueous solution, and treated with ultrasound (30~40 °C, 700 W) for 20 min. Then, 40 mL methanol was added for ultrasonic treatment for 50 min. Filtrate was obtained by filtration of 0.22 μm Millipore filter unit, and 10 μL of sample solution was injected into HPLC for determination.
According to method of Chen et al. [19 (link)], the samples were analyzed by HPLC (Waters) using C18 (4.6 × 250 mm, 5.0 μm, Waters E2695, Milford, MA, USA) at 30 °C, and the content of metabolites was determined: The mobile phase was methanol −0.1% phosphoric acid (80:20). The flow rate was 1 mL·min⁻¹. The detection wavelength was 254 nm.

Chen D., Jia L., Hou Q., Zhao X, & Sun K. (2021). Analysis of Endophyte Diversity of Rheum palmatum from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite. Microorganisms, 9(5), 978.

+ Open protocol

+ Expand

Quantitative Analysis of Medicinal Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standards of gentiopicroside, loganic acid, swertiamarine and sweroside were purchased from Shanghai R&D Center for Standardization of Traditional Chinese Medicines. High-performance liquid chromatography (HPLC)-ultrapure water, analytical-grade methanol and phosphoric acid were purchased from Sangon Biotech, Ltd. (Shanghai, China).
The dried plant samples (i.e., subset of the same tissue that was surface sterilized and used for DNA extraction) were pulverized and sieved through a 300 μm mesh. A total of 1.0 g of powdered samples were weighed and 20 mL methanol was added and treated with ultrasound (30–40 °C, 250 W, 50 kHZ) for 30 min. Filtrate was obtained by filtration of 0.22 μm Millipore filter unit, and 10 μL of sample solution was injected into HPLC for determination. Samples were analyzed by HPLC (Waters) using C18 (4.6 × 250 mm, 5.0 μm, Waters E2695; Milford, MA, USA) at 30 °C, and the content of metabolites were determined: The mobile phase was methanol (A) −0.15% phosphoric acid (B). 0–4 min, 25% A; 4–12 min, 25–33% A; 12–20 min, 33–40% A; 20–25 min, 40–25% A. The flow rate was 1 mL·min⁻¹. The detection wavelength was 242 nm.

Hou Q., Chen D., Wang Y.P., Ehmet N., Ma J, & Sun K. (2022). Analysis of endophyte diversity of Gentiana officinalis among different tissue types and ages and their association with four medicinal secondary metabolites. PeerJ, 10, e13949.

+ Open protocol

+ Expand

Quantitative Analysis of Gentianaceae Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard gentiopicroside, loganic acid, Swertiamarine and Sweroside were obtained from Shanghai R&D Center for Standardization of Traditional Chinese Medicines. High-performance liquid chromatography (HPLC)- ultrapure water, analytical-grade methanol and phosphoric acid were purchased from Sangon Biotech, Ltd. (Shanghai, China).
The dried root of each treatment specimen (three replications) was pulverized and sieved through a 300 μm mesh. A total of 1.0 of powder of each sample was precisely weighed and added 20 mL methanol, and treated with ultrasound (30 ~ 40 °C, 250 W, 50kHZ) for 30 min. Filtrate was obtained by filtration of 0.22 μm Millipore filter unit, and 10 μL of sample solution was injected into HPLC for determination.
The samples were analyzed by HPLC (Waters.) using C18 (4.6 × 250 mm, 5.0 μm, Waters E2695, USA) at 30 °C, and the content of metabolites was determined: The mobile phase was methanol (A) - 0.15% phosphoric acid (B). 0–4 min, 25% A; 4–12 min, 25 -33% A; 12–20 min, 33 - 40% A; 20–25 min, 40 - 25% A. The flow rate was 1 mL·min^− 1. The detection wavelength was 242 nm.

Hou Q.Z., Chen D.W., Wang Y.P., Ehmet N., Ma J, & Sun K. (2022). Analysis of endophyte diversity of two Gentiana plants species and the association with secondary metabolite. BMC Microbiology, 22, 90.

+ Open protocol

+ Expand

Quantification of Rhubarb Metabolites by HPLC

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard aloe-emodi, rhein, emodin, chrysophanol, and physcion were obtained from Shanghai R&D Center for Standardization of Traditional Chinese Medicines. High-performance liquid chromatography (HPLC)-ultrapure water, analytical-grade methanol, NaHCO3, and phosphoric acid were purchased from Sangon Biotech, Ltd. (Shanghai, China).
The metabolites of R. palmatum were measured according to the methods of Chen et al. [32 (link)]; the dried root of different treatments (five replicates) was pulverized and sieved by using a mesh (300 μm). An amount of 0.15 g of powder was precisely weighed, 10 mL 0.1% (w/v) NaHCO₃ solution was added, and ultrasonic treatment was performed (30–40 °C, 700 W) for 20 min. Then, 40 mL of methanol was added and treated using ultrasound for 50 min. The sample solution was filtered by a Millipore filter unit (0.22 μm), and then 10 μL of sample solution was injected into the HPLC for measurement.
HPLC system and parameters were set according to the methods of Chen et al. [33 (link)]; the metabolites content of samples was determined by HPLC (Waters E2695, Milford, MA, USA) using C18 (4.6 × 250 mm, 5.0 μm) at 30 °C. The mobile phase of HPLC was methanol −0.1% phosphoric acid (80:20); the flow rate was 1 mL/min. The detection wavelength was 254 nm.

Chen D., Shi W., Wang Y., Zhao J., Zhang H., Jia L, & Sun K. (2022). Molecular Traits Underlying the Growth Promotion and Metabolite Accumulation in Rheum palmatum Inoculated with Endophytic Trichoderma citrinoviride HT-1. International Journal of Molecular Sciences, 23(21), 13132.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!