Conventional office scanner

Manufactured by Epson

Sourced in Germany

The Epson conventional office scanner is a desktop device designed to digitize physical documents. It functions by optically scanning the contents of paper documents and converting them into digital image files, enabling users to archive, share, and manipulate the information electronically.

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Lab products found in correlation

Crystal violet, by Serva Electrophoresis (1 mentions) Methanol, by Carl Roth (1 mentions)

Close spelling variants of this product

Office scanner

2 protocols using conventional office scanner

Quantification of Colony Formation

Cited 1 time

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Cells were seeded at a density of 2 × 10³ cells/well in RPMI 1640 supplemented with 10% FBS, penicillin (100 u/mL), streptomycin (100 u/mL), and L-glutamine (2 mM) (Gibco, Thermo Fischer, Frankfurt, Germany). Cells were treated with increasing concentrations of A4B17 and cultured for 14 days. Medium and compounds were exchanged after 7 days. The formation of colonies was visualized using 0.5% crystal violet (w/v) (SERVA, Heidelberg, Germany) in 20% methanol (v/v) (Carl Roth, Karlsruhe, Germany). Plates were scanned in a conventional office scanner (Epson, Meerbusch, Germany). The area covered by colonies was calculated using the ColonyArea Plugin for ImageJ (Guzmán et al., 2014 (link)).

Kuznik N.C., Solozobova V., Lee I.I., Jung N., Yang L., Nienhaus K., Ntim E.A., Rottenberg J.T., Muhle-Goll C., Kumar A.R., Peravali R., Gräßle S., Gourain V., Deville C., Cato L., Neeb A., Dilger M., Cramer von Clausbruch C.A., Weiss C., Kieffer B., Nienhaus G.U., Brown M., Bräse S, & Cato A.C. (2022). A chemical probe for BAG1 targets androgen receptor-positive prostate cancer through oxidative stress signaling pathway. iScience, 25(5), 104175.

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Colony Formation Assay for X15695

Cited 1 time

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Cells were seeded at a density of 1–2 × 10³ cells/well in a 6-well plate and treated with increasing concentrations of X15695 and cultured for 14–21 days. Medium and compounds were exchanged after 7 days. Cells were fixed with methanol/acetic acid mixture [3:1 volume for volume (v/v)] and the formation of colonies was visualized using 0.5% crystal violet (w/v) in 20% methanol (v/v). Plates were scanned in a conventional office scanner (Epson). The area covered by colonies was calculated using the ColonyArea Plugin for ImageJ (RRID:SCR_003070; ref. 24 (link)).

Pan M., Solozobova V., Kuznik N.C., Jung N., Gräßle S., Gourain V., Heneka Y.M., Cramer von Clausbruch C.A., Fuhr O., Munuganti R.S., Maddalo D., Blattner C., Neeb A., Sharp A., Cato L., Weiss C., Jeselsohn R.M., Orian-Rousseau V., Bräse S, & Cato A.C. (2023). Identification of an Imidazopyridine-based Compound as an Oral Selective Estrogen Receptor Degrader for Breast Cancer Therapy. Cancer Research Communications, 3(7), 1378-1396.

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