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3 protocols using α ketoglutaric

1

GC-MS Analysis of Organic Acids

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The 14 OA standards (pyruvic, lactic, glycolic, 2-hydroxybutyric, 3-hydroxybutyric, malonic, succinic, fumaric, α-ketoglutaric, malic, 2-hydroxyglutaric, cis-aconitic, citric, and isocitric acid), and O-methoxyamine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) + 1% tert-butyldimetheylchlorosilane (TBDMCS) was obtained from Thermo Scientific (Bellefonte, PA, USA). Toluene, diethyl ether, ethyl acetate, and sodium chloride of pesticide grade were supplied by Kanto Chemical (Tokyo, Japan). All other chemicals were of analytical grade and used as received.
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2

GC-MS Analysis of Organic Acids

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The 14 OA standards (pyruvic, lactic, glycolic, 2-hydroxybutyric, 3-hydroxybutyric, malonic, succinic, fumaric, α-ketoglutaric, malic, 2-hydroxyglutaric, cis-aconitic, citric, and isocitric acid), and O-methoxyamine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) + 1% tert-butyldimetheylchlorosilane (TBDMCS) was obtained from Thermo Scientific (Bellefonte, PA, USA). Toluene, diethyl ether, ethyl acetate, and sodium chloride of pesticide grade were supplied by Kanto Chemical (Tokyo, Japan). All other chemicals were of analytical grade and used as received.
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3

Standardization of Flavonoids and Organic Compounds

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Standards of flavonoids (scutellarin, baicalin, baicalein, wogonin, wogonoside, luteolin, chrysin), verbascoside, sugars (glucose, fructose, xylose, galactose, rhamnose, sucrose and cellobiose) and organic acids (quinic, malic, ascorbic, succinic, citric, α-keto-glutaric, oxalic and tartaric), methanol and acetonitrile (HPLC gradient grade) were purchased from Sigma-Aldrich (Darmstadt, Germany). Water was obtained from Milipore Milli-Q Gradient water purification system (Barnstead, US).
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