Nucleospin plant 2 isolation kit

Samples of onions (roots, bulbs, and leaves) were crushed with a mortar and pestle in liquid nitrogen conditions. Each sample had a wet weight of 100 mg and was used for DNA isolation using a NucleoSpin Plant II isolation kit (Macherey-Nagel GmbH & Co. KG, Dueren, Germany) with miniprep protocol that was recommended by manufacturer using PL1 lysis buffer. Isolated DNA samples were used to determine the global DNA methylation status using a MethylFlash Methylated DNA Quantification Kit (Fluorometric) (Epigentek Group Inc., Farmingdale, NY, USA) and the manufacturer’s attached protocol, and 100 ng of DNA was used for each assay. Fluorescence measurement at 530_EX/590_EM nm was set on the fluorescence microplate reader (Tecan Infinity M200, Tecan Deutschland GmbH, Crailsheim, Germany) using Magellan software.

Roots and leaves of spinach plants were crushed with a mortar and pestle in liquid nitrogen conditions. Samples of 100 mg wet weight were used for DNA isolation using a NucleoSpin Plant II isolation kit (Macherey-Nagel GmbH & Co. KG, Dueren, Germany) with a miniprep protocol that was recommended by the manufacturer using a PL1 lysis buffer. Samples of isolated DNA were used for the global DNA methylation status determination with a MethylFlash Methylated DNA Quantification Kit (Fluorometric) (Epigentek Group Inc., Farmingdale, NY, USA) by the manufacturer’s recommended protocol, and 100 ng of DNA was used for each assay. Measurement of fluorescence at 530_EX/590_EM nm was set on the fluorescence microplate reader (Tecan Infinity M200, Tecan Deutschland GmbH, Crailsheim, Germany) and Magellan software for quantification.