Aposcreen annexin apoptosis kit

Manufactured by Southern Biotech

The ApoScreen Annexin Apoptosis kit is a laboratory equipment product that detects and quantifies apoptosis, a programmed cell death process, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a marker of apoptotic cells. The kit provides a straightforward method to assess apoptosis levels through fluorescence-based detection.

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Lab products found in correlation

Bkm120, by Selleck Chemicals (1 mentions)

Spelling variants of this product

ApoScreen Annexin V Apoptosis Kit (22 citations) Aposcreen Annexin V apoptosis kit-FITC (7 citations)

3 protocols using aposcreen annexin apoptosis kit

Apoptosis Induction Assay with EHT1864

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Cells were plated at 50,000/well in 12-well plates and treated with EHT1864 for 72 h. Twelve hours before analysis, positive and negative control wells were treated with or without 5 μM BKM120 (PI3K inhibitor, Selleck Chemicals), respectively. Floating and adherent cells (dislodged by trypsinization) were processed using ApoScreen Annexin Apoptosis kit (Southern Biotech) and analyzed by flow cytometry. Cells staining positively for Annexin-V were considered apoptotic.

Hampsch R.A., Shee K., Bates D., Lewis L.D., Désiré L., Leblond B., Demidenko E., Stefan K., Huang Y.H, & Miller T.W. (2017). Therapeutic sensitivity to Rac GTPase inhibition requires consequential suppression of mTORC1, AKT, and MEK signaling in breast cancer. Oncotarget, 8(13), 21806-21817.

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Annexin V Apoptosis Assay

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Cells seeded in triplicate in 6-well plates at 0.6-1×10⁶ cells/well were treated for 3-4 days as indicated in Fig. 2B. Floating and adherent cells (dislodged by trypsinization) were processed using ApoScreen Annexin Apoptosis kit (Southern Biotech), then analyzed by flow cytometry. Cells staining positively for Annexin-V and/or propidium iodide were considered apoptotic.

Bean J.R., Hosford S.R., Symonds L.K., Owens P., Dillon L.M., Yang W., Shee K., Schwartz G.N., Marotti J.D., Muller K.E., Rosenkranz K.M., Barth R.J., Chen V.S., Agarwal V.R, & Miller T.W. (2014). The PI3K/mTOR dual inhibitor P7170 demonstrates potent activity against endocrine-sensitive and endocrine-resistant ER+ breast cancer. Breast cancer research and treatment, 149(1), 69-79.

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Apoptosis Induction Quantification

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Cells seeded in triplicate in 6-well plates (5 × 10⁴ cells/well) were treated as indicated for 5 d. Floating and adherent cells (dislodged by trypsinization) were processed using the ApoScreen Annexin Apoptosis kit (Southern Biotech) and analyzed by flow cytometry with FlowJo software. Cells staining positively for Annexin V were considered apoptotic. For siRNA experiments, at 2 d after transfection, cells were treated with ligands/drugs as indicated for 3 d.

Shee K., Yang W., Hinds J.W., Hampsch R.A., Varn F.S., Traphagen N.A., Patel K., Cheng C., Jenkins N.P., Kettenbach A.N., Demidenko E., Owens P., Faber A.C., Golub T.R., Straussman R, & Miller T.W. (2018). Therapeutically targeting tumor microenvironment–mediated drug resistance in estrogen receptor–positive breast cancer. The Journal of Experimental Medicine, 215(3), 895-910.

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