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24sure cytochip

Manufactured by Illumina
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The 24Sure Cytochip is a laboratory equipment product designed for cytogenetic analysis. It is a microarray-based platform that enables the detection of chromosomal aberrations and abnormalities. The core function of the 24Sure Cytochip is to provide a comprehensive and accurate assessment of the entire chromosome complement in a single analysis.

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Lab products found in correlation

Innoscan 710, by Innopsys (3 mentions) Sureplex kit, by Illumina (3 mentions) Bluefuse multi analysis software, by Illumina (3 mentions) Fluorescence labelling system, by Illumina (1 mentions)

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24Sure (5 citations)

3 protocols using 24sure cytochip

1

Microarray-CGH Analysis of Single Cells

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Microarray-CGH analysis was undertaken according to our previously validated protocol using 24Sure Cytochip (Illumina, Cambridge, UK) (see online supplementary tables S5 and S6). Lysis and whole genome amplification of single cells biopsied from embryos were achieved using the SurePlex kit (Illumina). The entirety of this procedure took place according to the manufacturer's instructions. The fluorescence labelling system (Illumina) was used for the labelling of the amplified ethylenediaminetetraacetic acid-Tris (TE) samples and also for labelling a commercially available reference DNA (Illumina). Test TE samples were labelled with Cy3 while the reference 46,XY DNA was labelled with Cy5. Test and reference DNAs’ co-precipitation, their denaturation, array hybridisation and the posthybridisation washes all took place according to protocols provided by the manufacturer. The hybridisation time was 16 h.
A laser scanner (InnoScan 710, Innopsys, Carbonne, France) was used to analyse the microarrays after washing and drying. The resulting images were stored in TIFF format file and examined by the BlueFuse Multi analysis software (Illumina). Chromosome profiles were examined for gain or loss with the use of a 3× SD assessment.
Wells D., Kaur K., Grifo J., Glassner M., Taylor J.C., Fragouli E, & Munne S. (2014). Clinical utilisation of a rapid low-pass whole genome sequencing technique for the diagnosis of aneuploidy in human embryos prior to implantation. Journal of Medical Genetics, 51(8), 553-562.
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2

Whole Genome Analysis of ePNT Blastocysts

Cited 1 time
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Clumps of cells were harvested from ePNT blastocysts for whole genome amplification followed by microarray-CGH analysis according to a previously validated protocol using 24Sure Cytochip (Illumina, Cambridge, UK). Cells were obtained from the TE, ICM or both. Lysis and whole genome amplification was performed using the SurePlex kit (Illumina) according to the manufacturer’s instructions. Samples from ePNT blastocysts were labelled with Cy3 while a commercially available reference 46,XY DNA was labelled with Cy5 (Illumina)32 (link). A laser scanner (InnoScan 710, Innopsys, Carbonne, France) was used to analyse the microarrays after washing and drying. The resulting images were analysed using BlueFuse Multi analysis software (Illumina).
Hyslop L.A., Blakeley P., Craven L., Richardson J., Fogarty N.M., Fragouli E., Lamb M., Wamaitha S.E., Prathalingam N., Zhang Q., O’Keefe H., Takeda Y., Arizzi L., Alfarawati S., Tuppen H.A., Irving L., Kalleas D., Choudhary M., Wells D., Murdoch A.P., Turnbull D.M., Niakan K.K, & Herbert M. (2016). Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature, 534(7607), 383-386.
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3

Whole Genome Analysis of ePNT Blastocysts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Clumps of cells were harvested from ePNT blastocysts for whole genome amplification followed by microarray-CGH analysis according to a previously validated protocol using 24Sure Cytochip (Illumina, Cambridge, UK). Cells were obtained from the TE, ICM or both. Lysis and whole genome amplification was performed using the SurePlex kit (Illumina) according to the manufacturer’s instructions. Samples from ePNT blastocysts were labelled with Cy3 while a commercially available reference 46,XY DNA was labelled with Cy5 (Illumina)32 (link). A laser scanner (InnoScan 710, Innopsys, Carbonne, France) was used to analyse the microarrays after washing and drying. The resulting images were analysed using BlueFuse Multi analysis software (Illumina).
Hyslop L.A., Blakeley P., Craven L., Richardson J., Fogarty N.M., Fragouli E., Lamb M., Wamaitha S.E., Prathalingam N., Zhang Q., O’Keefe H., Takeda Y., Arizzi L., Alfarawati S., Tuppen H.A., Irving L., Kalleas D., Choudhary M., Wells D., Murdoch A.P., Turnbull D.M., Niakan K.K, & Herbert M. (2016). Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature, 534(7607), 383-386.
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