Sta compact analyzer

Manufactured by Diagnostica Stago

Sourced in France, United States

The STA Compact analyzer is a laboratory instrument designed for automated coagulation testing. It performs a range of coagulation assays, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen tests, among others. The STA Compact provides standardized and consistent results for these common coagulation measurements.

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Lab products found in correlation

Sta fibrinogen, by Diagnostica Stago (2 mentions) Cobas integra 400 plus analyzer, by Roche (1 mentions) Xt2000v, by Sysmex (1 mentions) Paxgene blood rna isolation kit, by Qiagen (1 mentions) Trizol reagent, by Merck Group (1 mentions) Sta ptt automate, by Diagnostica Stago (1 mentions) Sta neoplastine ci plus, by Diagnostica Stago (1 mentions) Lh750, by Beckman Coulter (1 mentions) Xe 2100, by Sysmex (1 mentions) Vacutainer tube, by BD (1 mentions) Dade actin fs, by Siemens (1 mentions) Sta r evolution analyzer, by Diagnostica Stago (1 mentions) Hemoclot, by Hyphen Biomed (1 mentions) Thromborel s, by Siemens (1 mentions)

9 protocols using sta compact analyzer

Measurement of Inflammatory and Coagulation Markers

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CRP was obtained from one of the lithium heparin aliquots using the Immulite 1000 CRP assay (Diagnostic Product Corporation, USA).
Fibrinogen. Fibrinogen levels were obtained from CTAD plasma. The 4.5 ml CTAD tube was stored during transport in melting ice and upon arrival at the laboratory, centrifuged for 20 minutes at 2000x g at 4° C, after which citrated plasma was harvested from the buffy coat and red blood cells, aliquoted (0.5 ml), snap frozen in dry ice, and stored at—30° C. One of these aliquots was used to obtain fibrinogen levels on a STA Compact Analyzer (Diagnostica Stago, France), using STA Fibrinogen (Diagnostica Stago, France).

Sirota M., Willemsen G., Sundar P., Pitts S.J., Potluri S., Prifti E., Kennedy S., Ehrlich S.D., Neuteboom J., Kluft C., Malone K.E., Cox D.R., de Geus E.J, & Boomsma D.I. (2015). Effect of Genome and Environment on Metabolic and Inflammatory Profiles. PLoS ONE, 10(4), e0120898.

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Standard Coagulation Assays Protocol

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PT and aPTT were measured in plasma using standard one-stage clotting assays (STA PT-Neoplastin CI, STA PTT- Automate, respectively) on the STA Compact analyzer (Diagnostica Stago, Parsippany, NJ, USA) according to manufacturer’s instructions.

Boothello R.S., Patel N.J., Sharon C., Abdelfadiel E.I., Morla S., Brophy D.F., Lippman H.R., Desai U.R, & Patel B.B. (2018). A Unique Non-Saccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase Activation.. Molecular cancer therapeutics, 18(1), 51-61.

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Clotting Time Assay for NSGM

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APTT and PT assays were performed in the standard one-stage format (STA PT-Neoplastin CI, STA PTT- Automate, respectively) on the STA Compact Analyzer (Diagnostica Stago, Parsippany, NJ, USA), according to manufacturer’s instructions.^{43 (link)} An aqueous solution of target NSGM (0 – 1 mM final concentration) was added to 300 μL of pooled human plasma from Valley Biomedical (Winchester, VA), incubated at 37°C for 5 min and the instrument operated in automated mode until collection of all clotting times. Experiments were performed in triplicate.

Morla S., Sankaranarayanan N.V., Afosah D.K., Kumar M., Kummarapurugu A.B., Voynow J.A, & Desai U.R. (2019). On the Process of Discovering Leads that Target the Heparin-Binding Site of Neutrophil Elastase in the Sputum of Cystic Fibrosis Patients. Journal of medicinal chemistry, 62(11), 5501-5511.

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Comprehensive Blood Analysis Protocol

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Complete blood counts, including leukocyte differentials (CBC/diff), were determined from blood samples collected in EDTA-coated blood tubes and analyzed using a Sysmex XT2000V (Sysmex America, Mundelein, IL). Biochemical analyses of serum samples collected in red-top Vacutainers were performed using the Roche COBAS Integra 400 plus analyzer (Roche Diagnostics, Indianapolis, IN). For coagulation studies, blood was collected in 3.2% sodium citrate-coated vacutainers and processed according to the manufacturer’s recommendations. Plasma from the citrate-coated tubes was analyzed on a STA Compact analyzer (Diagnostica Stago, Parsippany, NJ) for activated partial thromboplastin time (aPTT) and prothrombin time (PT) and for fibrinogen and D-dimer concentrations.

Wahl-Jensen V., Johnson J.C., Lauck M., Weinfurter J.T., Moncla L.H., Weiler A.M., Charlier O., Rojas O., Byrum R., Ragland D.R., Huzella L., Zommer E., Cohen M., Bernbaum J.G., Caì Y., Sanford H.B., Mazur S., Johnson R.F., Qin J., Palacios G.F., Bailey A.L., Jahrling P.B., Goldberg T.L., O’Connor D.H., Friedrich T.C, & Kuhn J.H. (2016). Divergent Simian Arteriviruses Cause Simian Hemorrhagic Fever of Differing Severities in Macaques. mBio, 7(1), e02009-15.

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Coagulation Factors Measurement Protocol

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Fibrinogen, PT, aPTT, and von Willebrand factor antigen (vWF) function were measured in plasma using standard assays (STA fibrinogen clotting activity assay, PT-Neoplastin CI, PTT- Automate, PTT CK Prest, and Liatest vWF assays, respectively) on the STA Compact analyzer (Diagnostica Stago, Parsippany, NJ, USA) according to manufacturer’s instructions.

Liebrecht L.K., Newton J., Martin E.J., Wickramaratne N., Jayaraman S., Han J., Aboutanos M., Brophy D.F, & Mangino M.J. (2019). Effects of a novel low volume resuscitation solutions on coagulation and platelet function. PLoS ONE, 14(5), e0215386.

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Comprehensive Thrombophilia Screening Protocol

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For each patient (median age, 31.5 years), the clinical profile and laboratory investigations (hemogram, PT, aPTT, Fibrinogen, D-dimer) were performed along with the complete thrombophilia screening including Protein C and S deficiency and Antithrombin-III deficiency. Protein C and Protein S activities were determined by using commercially available STACLOT kit on an automated STA compact analyzer (Diagnostica Stago). The quantification of AT III was done using STACHROM AT III kit in STA analyzer. Activated protein C resistance (APCR) was carried out using the Stago kit. PCR-RFLP method was used to perform mutational analysis for Factor V Leiden (1691G/A, rs6025), prothrombin (20210G/A, rs1799963), tissue factor plasmin inhibitor (-536C/T), fibrinogen-β (148C/T), MTHFR (677C/T) and plasminogen activator inhibitor-1 (16754G/5G) were performed by PCR-RFLP method. Total RNA from whole blood and plasma were isolated using PAXgene Blood RNA Isolation Kit (Qiagen) and TRIZOL reagent (Sigma) respectively, according to the manufacturer's instructions. miRNA expression and mRNA expression were analyzed as described earlier. Microparticle-TF activity assay was performed in human plasma using MP-TF assay kit (Aniara) according to the manufacturer's instructions.

Sahu A., Jha P.K., Prabhakar A., Singh H.D., Gupta N., Chatterjee T., Tyagi T., Sharma S., Kumari B., Singh S., Nair V., Goel S, & Ashraf M.Z. (2017). MicroRNA-145 Impedes Thrombus Formation via Targeting Tissue Factor in Venous Thrombosis. EBioMedicine, 26, 175-186.

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Fasting Blood Sampling for Metabolic Profile

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Blood samples were taken between 8.00 and 11.00 a.m. in a fasting state in order to monitor metabolic parameters. For blood counts, samples were obtained in EDTA-containing sterile tubes (S-Monovette® 1.2 mL, EDTA). Platelet counts were measured using Sysmex XE 2100, which has the capability to provide both an impedance platelet count and a fluorescence-based optical platelet count when the sample is run in the reticulocyte mode and Coulter LH-750, which uses impedance technology with enhanced data extraction techniques and algorithms to eliminate interference. For coagulation testing, samples were collected in citrate containing sterile tubes (S-Monovette® 1.4 mL, citrate). The activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen activity were measured on the STA-Compact analyzer (Diagnostica Stago, Paris, France) via mechanical clot detection using the following reagents: Sta-PTT Automate, Sta-Neoplastine CI plus, and Sta-Fibrinogen, all by Diagnostica Stago. Platelet counts are given in G/ L; APTT values in seconds; PT values are expressed as percentage of normal, fibrinogen activity given in mg/dL.

Dressler A., Chiara H., Benninger F., Waldhoer T., Gröppel G., Trimmel-Schwahofer P., Samueli S., Reithofer E., Elisa E., Feucht M, & Male C. (2020). Effects of the ketogenic diet on platelet counts and global coagulation tests in childhood epilepsy. Seizure, 80.

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Quantification of Apixaban and Anti-FXa Activity

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Apixaban concentration was determined using a validated liquid chromatography tandem mass spectroscopy method (Intertek Pharmaceutical Service, El Dorado Hills, CA). The lower limit of quantification was 1 ng/mL.23 Anti‐FXa activity was measured at Esoterix Coagulation Laboratory (Englewood, CO) using the Diagnostica Stago Rotachrom Heparin assay on a STA‐Compact analyzer (Diagnostica Stago, Parsippany, NJ)24 and is reported in low‐molecular‐weight heparin (LMWH) units (0.1 to 18 IU/mL).

Byon W., Sweeney K., Frost C, & Boyd R. (2017). Population Pharmacokinetics, Pharmacodynamics, and Exploratory Exposure–Response Analyses of Apixaban in Subjects Treated for Venous Thromboembolism. CPT: Pharmacometrics & Systems Pharmacology, 6(5), 340-349.

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Plasma Coagulation Tests for Dabigatran

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Blood was collected into a Vacutainer tube (Becton Dickinson Canada, Mississauga, Ontario, Canada) containing sodium citrate (0.105 M, 3.2%), centrifuged for 15 min at 1500 9g, and double-spun to remove platelets. Platelet-poor plasma was separated into aliquots and stored at À 70 °C. Plasma samples were analyzed at the Hamilton Regional Special Coagulation Laboratory by personnel who were blinded to patient characteristics.
The coagulation function tests (and assays) performed were: PT (Thromborel S; Siemens, Marburg, Germany); APTT (Dade Actin FS; Siemens ); TT (Sigma-Aldrich, Oakville, Ontario, Canada); and dTT (Hemoclot; Hyphen BioMed, Neuville-sur-Oise, France). The APTT and TT were measured on am STA Compact analyzer, and the dTT was measured on a STAR Evolution analyzer (both from Diagnostica Stago, Asni eres-sur-Seine, France). Plasma dabigatran levels were measured with a validated HPLC method (AAI Pharma Deutschland, Neu-Ulm, Germany) [30] . The results from these coagulation tests were not available for clinical use, but study participants could have clinically indicated laboratory testing, including coagulation tests.

Douketis J.D., Wang G., Chan N., Eikelboom J.W., Syed S., Barty R., Moffat K.A., Spencer F.A., Blostein M, & Schulman S. (2016). Effect of standardized perioperative dabigatran interruption on the residual anticoagulation effect at the time of surgery or procedure. Journal of thrombosis and haemostasis : JTH, 14(1).

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