3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt

SSM (purity ≥ 98%), DSS (MW ~40 kDa), L-buthionine-sulfoximine (BSO) (purity ≥ 97%), PD98059 (purity ≥ 98%), wortmannin (purity ≥ 98%), L-sulforaphane (SFN) (purity ≥ 95%), and 5-aminosalicylic acid (5-ASA) (purity ≥ 99%) were obtained from Sigma-Aldrich (St. Louis, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were from MP Biomedicals (CA, USA). Primary rabbit antibodies against Nrf2 (12721), Keap1 (8047), ERK1/2 (4695), p38 (8690), PKCα (59754), AKT (4685), p-p38 (Thr180/Tyr182) (4511), p-ERK1/2 (Thr202/Tyr204) (8544), p-AKT (Thr308) (13038), HO-1 (5853), lamin B (15068), and GAPDH (5174) were obtained from Cell Signaling Technology (MA, USA). Primary rabbit antibodies against NQO1 (GTX30626), GCLM (GTX53719), GCLC (GTX113197), and GR (GTX114199) were from GeneTex (Texas, USA). Primary rabbit antibody against p-PKCα (Thr638) (44-962G) was bought from Thermo Fisher Scientific (NH, USA). Goat anti-rabbit IgG antibody (D111018) was purchased from Sangon Biotech (Shanghai, China). Other chemicals were of analytical grade from commercial suppliers.

6-Aminonicotinamide (6-AN) and N-Acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Cis-diamminedichloroplatinum (II) (cisplatin, CDDP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Dimethyl sulfoxide (DMSO) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from MP Biomedicals (Santa Ana, CA, United States). Rabbit polyclonal anti-G6PD (8866), anti-PGD (13389), anti-TKT (8616) and anti-GAPDH (8884) were purchased from Cell Signaling Technology (Danvers, MA, United States), Rabbit polyclonal anti-TALDO1 (D123398) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Other chemicals were of analytical grade from commercial suppliers.