To investigate neurite growth cone sensing of biophysical cues, photopolymerization was used to generate micropatterned surfaces with various widths and depths as described elsewhere 12 (link),14 (link),16 (link). Briefly, the monomer mixtures of 39 wt% hexyl methacrylate and 60 wt% 1,6-hexanediol dimethacrylate (Sigma-Aldrich, St. Loius, MO) with 1 wt% of 2,2-dimethoxy-2-phenylacetophenone (Ciba, Tarrytown, NY) as the photoinitiator were spread on glass microscope slides and subsequently covered by photomasks with alternating transparent or reflective bands with a periodicity of 50 μm. Monomer samples were cured under a high pressure mecury vapor arc lamp (Omnicure S-1500) equipped with a homogenizing light pipe and collimating adapter. Radiation dosage was varied by altering UV exposure time to attain specific microfeature depths as previously described 12 (link),14 (link). Micropatterns substrates consisted of parallel ridges and grooves with 1–3 μm amplitudes and 50 μm periodicities for neurite contact guidance studies. Channel amplitudes and periodicities were characterized by white light interferometry using a Wyko NT 1100 optical profiling system (Veeco, Plainview, NY).
1 6 hexanediol dimethacrylate
Manufactured by Merck Group
Sourced in United States
1,6-hexanediol dimethacrylate is a chemical compound used as a crosslinking agent in various applications. It consists of two methacrylate functional groups attached to a hexane diiol backbone.
Automatically generated - may contain errors
Lab products found in correlation
Methyl methacrylate, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Nt1100, by Veeco (1 mentions)
Methyl acrylate, by Merck Group (1 mentions)
Lauroyl peroxide, by Merck Group (1 mentions)
2 hydroxy 2 methylpropiophenone, by Merck Group (1 mentions)
Butyl acrylate, by Merck Group (1 mentions)
Anhydrous methanol, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Methacrylic acid, by Merck Group (1 mentions)
N n dimethylacetamide, by Merck Group (1 mentions)
Tetrabutylammonium bromide, by Merck Group (1 mentions)
Anisole, by Merck Group (1 mentions)
Potassium tert butoxide, by Merck Group (1 mentions)
Basic alumina, by Merck Group (1 mentions)
N butyl acrylate, by Merck Group (1 mentions)
Phenylbis 2 4 6 trimethylbenzoyl phosphine oxide, by Merck Group (1 mentions)
Copper 2 bromide, by Merck Group (1 mentions)
6 protocols using 1 6 hexanediol dimethacrylate
1
Photopolymerized Micropatterns for Neurite Guidance
2
Raman Characterization of Liquid Resins
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A custom-built Raman upright microscope
with an excitation source (Kr + laser Innova 90-K, Coherent Inc.,
Santa Clara, USA; wavelength 647 nm; power 35 mW) was used
for the characterization of the liquid resins; corresponding untreated
and treated tiles; corresponding condensed liquids; BAPO and TPO-L
untreated, treated with only UV, only heat or both UV then heat; their
condensed liquids; methyl methacrylate; and (1,6)-hexanediol di-methacrylate
(Sigma-Aldrich). The excitation beam was focused onto the tiles, about
5 to 70 μm below the surface. The liquid samples (40 μL)
were placed in a cavity in a borosilicate glass slide. Scattered photons
were collected for 100 ms and focused on a 15 μm
pinhole at the entrance of a custom-made spectrograph, dispersing
them in the range of −40 till +3655 rel·cm–1. Spectral data were recorded with an EMCCD camera (Newton DU-970N-BV,
Andor Technology Ltd., Belfast, Northern Ireland), with an average
spectral resolution of 2.3 cm–1. By applying a raster
scan pattern covering a 30 × 30 μm2 area, 3600
spectra were recorded for each sample. Baseline correction was applied
for the treated industrial red and GL4000 resins that gave rise to
significant autofluorescence.
with an excitation source (Kr + laser Innova 90-K, Coherent Inc.,
Santa Clara, USA; wavelength 647 nm; power 35 mW) was used
for the characterization of the liquid resins; corresponding untreated
and treated tiles; corresponding condensed liquids; BAPO and TPO-L
untreated, treated with only UV, only heat or both UV then heat; their
condensed liquids; methyl methacrylate; and (1,6)-hexanediol di-methacrylate
(Sigma-Aldrich). The excitation beam was focused onto the tiles, about
5 to 70 μm below the surface. The liquid samples (40 μL)
were placed in a cavity in a borosilicate glass slide. Scattered photons
were collected for 100 ms and focused on a 15 μm
pinhole at the entrance of a custom-made spectrograph, dispersing
them in the range of −40 till +3655 rel·cm–1. Spectral data were recorded with an EMCCD camera (Newton DU-970N-BV,
Andor Technology Ltd., Belfast, Northern Ireland), with an average
spectral resolution of 2.3 cm–1. By applying a raster
scan pattern covering a 30 × 30 μm2 area, 3600
spectra were recorded for each sample. Baseline correction was applied
for the treated industrial red and GL4000 resins that gave rise to
significant autofluorescence.
3
Synthesis of Photopolymer Resin Components
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1,6-Hexanediol dimethacrylate (≥90%),
bis[2-(methacryloyloxy)ethyl] phosphate, butyl acrylate (≥99%),
methyl acrylate (99%), 2-hydroxy-2-methylpropiophenone (97%), sodium
hydroxide (NaOH, ≥ 98%), acetone, and lauroyl peroxide (98%)
were purchased from Sigma-Aldrich and used as received.
bis[2-(methacryloyloxy)ethyl] phosphate, butyl acrylate (≥99%),
methyl acrylate (99%), 2-hydroxy-2-methylpropiophenone (97%), sodium
hydroxide (NaOH, ≥ 98%), acetone, and lauroyl peroxide (98%)
were purchased from Sigma-Aldrich and used as received.
4
Photoinitiated Graft Polymerization
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1,6-Hexanediol
dimethacrylate (>90%), phenylbis(2,4,6-trimethylbenzoyl)phosphine
oxide (BAPO, 97%), ethyl α-boromoisobutyrate (EBiB, 98%), ethylene
bis(2-bromoisobutyrate) (2-BiB, 97%), copper(II) bromide (CuBr2, 99.999%), tris[2-(dimethylamino)ethyl]amine
(Me6TREN), methacrylic acid (99%), potassium tert-butoxide (potassium t-butoxide, 98%), and tetrabutylammonium
bromide (TBAB, 98%) were used as received from Sigma-Aldrich. n-Butyl acrylate (n-BA, 99%) was obtained
from Sigma-Aldrich and purified by passing through a column of basic
alumina (Sigma-Aldrich, activated, basic, Brockman I) to remove inhibitor.
Acetonitrile, anhydrous methanol, dichloromethane, acetone, anisole,
and N,N-dimethylacetamide were used
as received from Sigma-Aldrich. In addition, α,ω-methacryloxypropyl-terminated
poly(dimethylsiloxane) (DMS-R18, average molar mass of 5000 g/mol, Đ = 1.15) was obtained from Gelest and purified using
basic alumina columns to remove inhibitor.
dimethacrylate (>90%), phenylbis(2,4,6-trimethylbenzoyl)phosphine
oxide (BAPO, 97%), ethyl α-boromoisobutyrate (EBiB, 98%), ethylene
bis(2-bromoisobutyrate) (2-BiB, 97%), copper(II) bromide (CuBr2, 99.999%), tris[2-(dimethylamino)ethyl]amine
(Me6TREN), methacrylic acid (99%), potassium tert-butoxide (potassium t-butoxide, 98%), and tetrabutylammonium
bromide (TBAB, 98%) were used as received from Sigma-Aldrich. n-Butyl acrylate (n-BA, 99%) was obtained
from Sigma-Aldrich and purified by passing through a column of basic
alumina (Sigma-Aldrich, activated, basic, Brockman I) to remove inhibitor.
Acetonitrile, anhydrous methanol, dichloromethane, acetone, anisole,
and N,N-dimethylacetamide were used
as received from Sigma-Aldrich. In addition, α,ω-methacryloxypropyl-terminated
poly(dimethylsiloxane) (DMS-R18, average molar mass of 5000 g/mol, Đ = 1.15) was obtained from Gelest and purified using
basic alumina columns to remove inhibitor.
5
Capillary-Based Protein Separation and Analysis
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Fused-silica capillary tubings of 0.250, 0.200 and 0.075 mm I.D. (0.375 mm O.D.) with a polyimide outer coating were purchased from Polymicro Technologies (Phoenix, AZ, USA). Dorica Supporting devices (figure S1) to protect the capillary columns were from Avantech Group s.r.l (Angri, SA, Italy). Azoisobutyronitrile (AIBN), acetonitrile (ACN), trifluoroacetic acid (TFA), lauryl methacrylate (LMA), 1,6-hexanediol dimethacrylate (HDDMA), tert-butyl alcohol, 1,4butanediol, tetrahydrofuran (THF), methacrylic anhydride, pyridine, sodium hydroxide (NaOH), hydrochloric acid (HCl), ammonium bicarbonate, uracil, phenol, benzaldehyde, nitro-benzene, benzene, toluene, ethyl-benzene, n-propyl-benzene, n-butyl-benzene, n-pentyl-benzene as well as lysozyme from chicken egg white, -lactalbumin from bovine milk, -lactoglobulin B from bovine milk, carbonic anhydrase from bovine erythrocytes, core histones from calf thymus were purchased from Sigma-Aldrich (St. Louis, MO, USA). Porcine trypsin was purchased from Promega (Madison, WI, USA). (N-trimethoxysilylpropyl)-polyethylenimine from United Chemical Technologies (Bristol, PA, USA) was used as a silanization reagent.
6
Synthesis and Characterization of Methacrylate-based Polymers
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Methyl methacrylate (MMA, 99 %), ethylene glycol dimethacrylate (EGDMA, 98 %), and 1,6-hexanediol dimethacrylate (1,6-HDDMA, ≥90 %) were all purchased from Sigma-Aldrich (Oakville, ON Canada), and filtered over alumina to remove inhibitors. Diallyldimethylammonium chloride (DADMAC, 65 wt% in H 2 O), 2,2-dimethoxy-2-phenylacetophenone (DMPAP, 99 %), ethylene glycol diacrylate (EGDA, 90 %), methanol (≥99.9 % Chromasolv), potassium bromide (ACS reagent, 99.0 % , KBr), and fumed silica (powder, 0.2-0.3 µm average particle size) were purchased from Sigma-Aldrich and were used without further purification. iTaq polymerase, 10X PCR buffer, and magnesium chloride were obtained from Bio-Rad (Montreal, QC Canada). Primers and dNTP mix (dATP, dCTP, dGTP and dTTP) were obtained from Sigma-Aldrich. Ethidium bromide (UltraPure 10 mg/mL, EtBr) was purchased from Life Technologies Inc. (Burlington, ON Canada). A 100 bp DNA ladder was purchased from BioLabs (Ipswich, MA).
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