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Albuwell and creatinine companion

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The Albuwell and Creatinine Companion are analytical instruments used for the measurement of albumin and creatinine levels in biological samples. These devices provide quantitative analysis of these analytes, which are commonly used as indicators of kidney function and overall health.

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Lab products found in correlation

Ab211094, by Abcam (1 mentions) Autokit glucose, by Fujifilm (1 mentions)

Spelling variants of this product

Creatinine Companion (49 citations) Albuwell (16 citations) Creatinine (4 citations) Albuwell M and Creatinine Companion kits (4 citations) Albuwell and Creatinine companion kit (3 citations)

4 protocols using albuwell and creatinine companion

1

Urinary Biomarker Quantification in Mice

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Urine samples were collected from mice individually housed in metabolic cages. Urinary albumin level was estimated by Coomassie Blue gel staining of electrophoresed urine samples, as reported previously [17] (link). In brief, 5 μl urine samples from individual mice were temperature denatured and separated using a 10% SDS-PAGE gel and a mouse albumin standard (mouse serum albumin; Exocell, Philadelphia, PA, USA), then stained with Coomassie Blue.
The albumin/creatinine ratio (ACR) (mg/mmol) (Albuwell and Creatinine Companion, Exocell, Philadelphia, PA, USA), urinary angiotensin II (Ang II)/creatinine ratio (pmol/μmol) (Immuno-Biological Laboratories, IBL America, Minneapolis, MN, USA), and angiotensin 1-7 (Ang 1-7)/ creatinine ratio (pmol/μmol) (Immuno-Biological Laboratories, IBL America, Minneapolis, MN, USA), were assayed by ELISA and normalised by urinary creatinine levels as described [34] (link)[35] (link)[36] (link).
Liao M.C., Pang Y.C., Chang S.Y., Zhao X.P., Chenier I., Ingelfinger J.R., Chan J.S.D, & Zhang S.L. (2021). AT2R deficiency in mice accelerates podocyte dysfunction in diabetic progeny in a sex-dependent manner. Diabetologia, 64(9).
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2

Urinary Biomarkers in Murine Nephropathy

Cited 1 time
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Urine samples were collected from mice individually housed in metabolic cages. Urinary albumin/creatinine ratio (mg/mmol) (Albuwell and Creatinine Companion, Exocell Inc., Philadelphia, PA, USA) was determined biweekly post-ADR injection by ELISA and normalized by urinary creatinine levels as described [10 (link), 34 (link)]. Urinary albumin level was also estimated by Coomassie Blue gel staining of electrophoresed urine samples as reported.[10 (link), 34 (link)] Urinary Ang II /creatinine ratio (pmol/μmol) (Immuno-Biological Laboratories, IBL America, Minneapolis, MN, USA) was assayed by ELISA and normalized by urinary creatinine levels as described.[34 (link), 35 (link)]
Liao M.C., Miyata K.N., Chang S.Y., Zhao X.P., Lo C.S., El-Mortada M.A., Peng J., Chenier I., Yamashita M., Ingelfinger J.R., Chan J.S, & Zhang S.L. (2022). Angiotensin II Type-2-Receptor Stimulation Ameliorates Focal and Segmental Glomerulosclerosis in Mice. Clinical science (London, England : 1979), 136(10), 715-731.
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3

Histological and Functional Assessment of Diabetic Nephropathy

Cited 1 time
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Kidney sections were stained with Periodic-Acid Schiff (PAS) and Masson’s trichrome to reveal renal morphologic changes23 (link),48 (link). The changes of DN features—glomerulosclerosis (based on PAS images, scale from 0 to 4) and glomerular fibrosis (based on Masson staining) were scored with the scorer blinded to the group23 (link),48 (link). Relative staining was quantified with NIH Image J software (Bethesda, MD, USA). The images (N = 6~10 per animal, 6–11 mice/group) were analyzed and quantitated in a blinded fashion23 (link),48 (link). Glomerular filtration rate (GFR) was measured in conscious mice by the fluorescein isothiocyanate-inulin method as reported previously23 (link),49 (link), as recommended by the Diabetic Complications Consortium (http://www.diacomp.org/). Urine samples, collected from mice individually housed in metabolic cages, were assayed for albumin/creatinine ratio (ACR) (Albuwell and Creatinine Companion, Exocell Inc., Philadelphia, PA, USA)23 (link),49 (link).
Zhao X.P., Chang S.Y., Liao M.C., Lo C.S., Chenier I., Luo H., Chiasson J.L., Ingelfinger J.R., Chan J.S, & Zhang S.L. (2018). Hedgehog Interacting Protein Promotes Fibrosis and Apoptosis in Glomerular Endothelial Cells in Murine Diabetes. Scientific Reports, 8, 5958.
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4

Comprehensive Kidney Function Evaluation

Cited 2 times
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Haematocrit was determined using glass microcapillaries at the first time point of inulin clearance. Urinary albumin concentration was measured by ELISA (Albuwell and Creatinine Companion, Exocell, Philadelphia, PA, USA)[13 (link)–15 (link), 18 (link), 22 (link)], and 24-h urinary albumin excretion was calculated with the urine volume. Serum and urine glucose concentrations were determined using a colorimetric-enzymatic method (Autokit Glucose, Wako Diagnostics, Richmond, VA, USA). Fractional reabsorption of glucose (FR-glucose) was calculated using the serum and urine glucose concentration, urine volume, and GFR of each mouse [23 (link)]. Urinary Ang II levels were measured by a commercial ELISA kit (Bachem America, Torrence, CA, USA) according to the protocol III as described previously [15 (link), 18 (link)]. Urinary adenosine was assayed by fluorometric method (Abcam (Cambridge, MA, USA), catalogue no. ab211094). The urine was pre-treated with catalase beads (Abcam, catalogue no. 218718) and the fluorescence signal was detected by excitation 535nm and emission 587nm.
Miyata K.N., Lo C.S., Zhao S., Zhao X.P., Chenier I., Yamashita M., Filep J.G., Ingelfinger J.R., Zhang S.L, & Chan J.S. (2021). Deletion of heterogeneous nuclear ribonucleoprotein F in renal tubules downregulates SGLT2 expression and attenuates hyperfiltration and kidney injury in a mouse model of diabetes. Diabetologia, 64(11), 2589-2601.
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