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Pe cy7 rat anti mouse cd31 clone 390

Manufactured by BD
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PE-Cy7 rat anti-mouse CD31 (clone 390) is a fluorescently-labeled antibody that binds to the CD31 (PECAM-1) cell surface antigen expressed on mouse endothelial cells.

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Lab products found in correlation

Facsaria 2 sorp, by BD (2 mentions) Streptavidin pe, by BD (2 mentions) Biotin rat anti mouse cd106 clone 429, by BD (2 mentions) Pe cy7 rat anti mouse cd45 clone 30 f11, by BD (2 mentions)

Close spelling variants of this product

PE-Cy7 anti-mouse CD31 (clone 390; PE-Cy7 Rat Anti-Mouse CD31 PE-Cy7 anti-mouse CD31 PE rat anti-mouse CD31 CD31 (clone 390, Anti-CD31 PE-Cy7 Rat anti-mouse CD31 CD31-PE-Cy7 Rat anti-CD31 Anti-CD31-PE

2 protocols using pe cy7 rat anti mouse cd31 clone 390

1

Satellite Cell Identification in Muscle

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscle samples were stained using an antibody mixture of PE-Cy7 rat anti-mouse CD31 (clone 390), PE-Cy7 rat anti-mouse CD45 (clone 30-F11), Biotin rat anti-mouse CD106 (clone 429(MVCAM.A)) and PE Streptavidin from BD Biosciences (San Diego, CA); and Itga7 647 (clone R2F2) from AbLab (Vancouver, B.C., Canada). Antibody cocktail was added to samples and incubated on ice for 30 min. Samples were washed and resuspended with FACS staining media containing propidium iodide for FACS analysis on the FACSAriaII SORP (BD Biosciences, San Diego, CA). Total satellite cells (lineage negative; VCAM, alpha7 double positive cells or ZsGreen+) (Figures S1A and S1B) were analyzed while draining the entire sample from each skeletal muscle sample. For transplanted tibialis anterior (TA) muscles, the number of donor (ZsGreen + or tdTOM+) satellite cells were examined as previously described (Arpke et al., 2013 (link)).
Collins B.C., Arpke R.W., Larson A.A., Baumann C.W., Xie N., Cabelka C.A., Nash N.L., Juppi H.K., Laakkonen E.K., Sipilä S., Kovanen V., Spangenburg E.E., Kyba M, & Lowe D.A. (2019). Estrogen Regulates the Satellite Cell Compartment in Females. Cell reports, 28(2), 368-381.e6.
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2

Satellite Cell Identification in Muscle

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscle samples were stained using an antibody mixture of PE-Cy7 rat anti-mouse CD31 (clone 390), PE-Cy7 rat anti-mouse CD45 (clone 30-F11), Biotin rat anti-mouse CD106 (clone 429(MVCAM.A)) and PE Streptavidin from BD Biosciences (San Diego, CA); and Itga7 647 (clone R2F2) from AbLab (Vancouver, B.C., Canada). Antibody cocktail was added to samples and incubated on ice for 30 min. Samples were washed and resuspended with FACS staining media containing propidium iodide for FACS analysis on the FACSAriaII SORP (BD Biosciences, San Diego, CA). Total satellite cells (lineage negative; VCAM, alpha7 double positive cells or ZsGreen+) (Figures S1A and S1B) were analyzed while draining the entire sample from each skeletal muscle sample. For transplanted tibialis anterior (TA) muscles, the number of donor (ZsGreen + or tdTOM+) satellite cells were examined as previously described (Arpke et al., 2013 (link)).
Collins B.C., Arpke R.W., Larson A.A., Baumann C.W., Xie N., Cabelka C.A., Nash N.L., Juppi H.K., Laakkonen E.K., Sipilä S., Kovanen V., Spangenburg E.E., Kyba M, & Lowe D.A. (2019). Estrogen Regulates the Satellite Cell Compartment in Females. Cell reports, 28(2), 368-381.e6.
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