Log In Sign up
The largest database of trusted experimental protocols

Guavasoft version 3

Manufactured by Merck Group
Visit Supplier
Sourced in United States

GuavaSoft is a software package developed by Merck Group for laboratory data analysis. Version 3.1.1 includes enhancements to the core data processing and visualization functions.

Automatically generated - may contain errors

Lab products found in correlation

Countbright absolute counting beads, by Thermo Fisher Scientific (2 mentions) Qifikit, by Agilent Technologies (2 mentions) Cytoflex, by Beckman Coulter (2 mentions) Fortessa, by BD (2 mentions) Canto 2, by BD (2 mentions)

3 protocols using guavasoft version 3

1

Quantitative Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was carried out according to previously published protocols (28 ). For cell number quantification CountBright® absolute counting beads (Life Technologies) were added. Samples were analyzed with flow cytometers from BD, Canto II and Fortessa (BD Bioscience, Germany) and a Beckman Coulter CytoFLEX for the long-term cultures. Surface antigen density of cell lines and constructs was evaluated with QIFIKIT (Agilent Dako, Santa Clara, CA, USA). Flow cytometry data was analyzed with FlowJo V10.3 software or GuavaSoft, version 3.1.1 (Merck Millipore). Staining approach outlined in supplementary methods.
Benmebarek M.R., Cadilha B.L., Herrmann M., Lesch S., Schmitt S., Stoiber S., Darwich A., Augsberger C., Brauchle B., Rohrbacher L., Oner A., Seifert M., Schwerdtfeger M., Gottschlich A., Rataj F., Fenn N.C., Klein C., Subklewe M., Endres S., Hopfner K.P, & Kobold S. (2021). A modular and controllable T cell therapy platform for acute myeloid leukemia. Leukemia, 35(8), 2243-2257.
+ Open protocol
+ Expand
2

Carboplatin-Induced Apoptosis Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of GSH used was 5 mM. When the cells (5×105 cells/well) were at 90% confluence, the samples were treated by carboplatin with or without GSH at 37°C for 12 h. Following cultivation with CBDCA with or without GSH, cells were stained with Hoechst 33342/PI and observed by confocal microscopy as previously described (24 (link)). CBDCA-induced apoptosis was quantified according to the percentage of Hoechst 33342 and/or PI-positive stained cells by guavaSoft version 3.11 (EMD Millipore, Billerica, MA, USA).
Shen B., Mao W., Ahn J.C., Chung P.S, & He P. (2018). Mechanism of HN-3 cell apoptosis induced by carboplatin: Combination of mitochondrial pathway associated with Ca2+ and the nucleus pathways. Molecular Medicine Reports, 18(6), 4978-4986.
+ Open protocol
+ Expand
3

Flow Cytometry Protocol for Cell Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was carried out according to previously published protocols [28 (link)]. For cell number quantification CountBright® absolute counting beads (Life Technologies) were added. Samples were analyzed with flow cytometers from BD, Canto II and Fortessa (BD Bioscience, Germany) and a Beckman Coulter CytoFLEX for the long-term cultures. Surface antigen density of cell lines and constructs was evaluated with QIFIKIT (Agilent Dako, Santa Clara, CA, USA). Flow cytometry data were analyzed with FlowJo V10.3 software or GuavaSoft, version 3.1.1 (Merck Millipore). Staining approach outlined in supplementary methods.
Benmebarek M.R., Cadilha B.L., Herrmann M., Lesch S., Schmitt S., Stoiber S., Darwich A., Augsberger C., Brauchle B., Rohrbacher L., Oner A., Seifert M., Schwerdtfeger M., Gottschlich A., Rataj F., Fenn N.C., Klein C., Subklewe M., Endres S., Hopfner K.P, & Kobold S. (2021). A modular and controllable T cell therapy platform for acute myeloid leukemia. Leukemia, 35(8), 2243-2257.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!