TEM was carried out for ENMs on an EM208, operating at 200 kV (Philips, Eindhoven, The Netherlands), with a high definition acquisition system based on a side-mounted TEM camera OSIS Morada and an iTEM soft-ware platform (Olympus Soft Imaging Solutions GmbH, Münster, Germany). ENMs, dispersed in MilliQ water, were placed onto a carbon-coated grid and dried at room temperature under vacuum.
Item software platform
Manufactured by Olympus
Sourced in Germany
The ITEM software platform is a comprehensive data management solution designed to streamline laboratory operations. It provides a centralized system for organizing and analyzing experimental data, enabling efficient data collection, storage, and retrieval.
Automatically generated - may contain errors
3 protocols using item software platform
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TEM Imaging of Engineered Nanomaterials
2
Characterization of Polyvinylpyrrolidone-Stabilized Silver Nanoparticles
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Polyvinylpirrolidone-stabilized AgNPs (content of silver: 25% w/w, polymer 75%) were supplied by NanoAmor Materials Inc. (Houston, TX, USA). AgNPs from the same batch were characterized and used in previous permeation studies through ex vivo human skin 18 and through porcine oromucosal membrane. 19 Size and morphology of the AgNPs metal cores, while dispersed in physiological solution, were obtained by means of Transmission Electron Microscopy (EM208; Philips, Eindhoven, The Netherlands, operating at 200 kV) with an high definition acquisition system, based on a sidemounted TEM camera (OSIS Morada) and an iTEM software platform (Olympus Soft Imaging Solutions GmbH, Münster, Germany). The polymer coating composition was confirmed using a FT-IR Spectrum 100 (Perkin Elmer, Waltham, MA, USA; FT-IR, Fourier transform-infrared) fitted with an attenuated total reflection (ATR) (Ge/Ge) accessory. 18 The average values of the AgNPs size and polydispersity, defined as a relative width of the size distribution, were determined from Dynamic Light Scattering (DLS) measurements, using a Zeta sizer Nano Z (Malvern Instruments Ltd.) analyzer, applying a 633 nm laser with a 173° orientation with respect to the sample. 19 Zeta potential measurements were carried out using a ZetasizerNano ZS (Malvern). The zeta potential was calculated using Henry's equation. 19
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Characterization of AgNPs in Solution
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AgNPs dispersed in physiological solution were characterized to obtain nanoparticles size and morphology on a transmission electron microscope (EM208; Philips, Eindhoven, The Netherlands operating at 200 kV) with an high definition acquisition system based on a side-mounted TEM camera OSIS Morada and a iTEM software platform (Olympus Soft Imaging Solutions GmbH, Münster, Germany).
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