Mouse cytokeratin 18 m30 elisa kit

Blood samples from fasting rats were collected from the cava vein at sacrifice. Glucose, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides, and albumin were measured with standard methods at the Hospital Vall d’Hebron CORE lab. Insulin was measured using an ELISA kit following the manufacturer’s instructions (EMD Millipore, Billerica, MA, USA). Insulin resistance was estimated by applying the homeostasis model of insulin resistance index (HOMA-IR): (fasting insulin (ng/mL) × fasting glucose (mg/dL))/405.
In the STAM™ mice, non-fasting blood was drawn from the facial vein for the quantification of glycated hemoglobin (HbA1c), cytokeratin (CK)-18, and biochemistry. HbA1c levels were quantified in whole blood by DCN2000 + (Siemens Healthcare Diagnostics, USA). Serum CK-18 levels were measured by using the Mouse Cytokeratin 18-M30 ELISA kit (Cusabio Biotech Co., Ltd., Wuhan, China). Serum alanine aminotransferase (ALT) and triglyceride levels were measured by FUJI DRI-CHEM 7000 (Fujifilm Corporation, Tokyo, Japan). Serum total cholesterol, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol levels were quantified by HPLC at Skylight Biotech Inc. (Akita, Japan).

The plasma Fgf21 concentration was determined using Rat/Mouse FGF-21 ELISA kits (Milipore, cat #EZRMFGF21-26K), according to the manufacturer’s instructions. Plasma insulin was measured using a Mouse Adipokine (MADKMAG-71K) kit (Millipore, Billerica, MA, USA), according to the manufacturer’s instructions. The cytokeratin-18 plasma concentration was measured using Mouse Cytokeratin 18-M30 ELISA kit (Cusabio, Hubei, China).