Hoechst 33342 staining
Hoechst 33342 is a fluorescent dye that binds to DNA. It can be used to stain and visualize DNA in various applications, such as flow cytometry and fluorescence microscopy.
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21 protocols using hoechst 33342 staining
Hoechst 33342 Staining for Cell Death
Quantifying Apoptosis in T24 Cells
High-Content Screening of E-Liquid Toxicity
Quantification of Histone H3 Citrullination
Quantification of Histone H3 Citrullination
Immunohistochemical Analysis of Biopsies
Multiparametric Imaging of SARS-CoV-2 Infection
Immunofluorescent Imaging of Macrophages and Dendritic Cells
Immunostaining of Anorectum Tissue
Mitochondrial Dynamics in Cardiomyocytes
After washing with PBS, cardiomyocytes were fixed with 4% paraformaldehyde for 15 min and incubated with 0.3% Triton X-100 for 10 min at room temperature; cardiomyocytes were then incubated with 1 μM MitoTracker Red (Invitrogen, United States) for 30 min followed by 10 μg/ml Hoechst 33342 staining for 10 min. Mitochondrial morphology was detected using an ultra-high-resolution laser scanning microscope (Olympus, Japan).
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