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2 protocols using fiske subbarow reducer reagent

1

Chitosan-Based Antimicrobial Delivery System

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Soy phosphatidylcholine (SPC; Lipoid S100, Lipoid GmbH, Ludwigshafen, Germany) was a generous gift from Lipoid GmbH. Chitosan (77% degree of deacetylation (DD), Fiske-SubbaRow reducer reagent, metronidazole, methanol, n-propanol, phosphorus standard, and Triton X solution were purchased from Sigma Aldrich Inc. (St. Luis, MO, USA). Ammonium molybdate and peroxide were purchased from Merck KGaA (Darmstadt, Germany), while sulfuric acid was purchased from May and Baker LTD (Dagenham, UK). Potato dextrose broth was purchased from Difco (BD, Franklin Lakes, NJ, USA) All other chemicals used in the experiments were of analytical grade. Carbopol®974P NF was a product from Lubrizol, Billingham, UK.
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2

Lipid-based drug delivery protocol

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Lipoid S 100 (phosphatidylcholine from soybean, >94% pure; PC) was a gift from Lipoid GmbH (Ludwigshafen, Germany). Calcein, rhodamine B, methanol CHROMASOLV ® , magnesium sulfate heptahydrate, potassium chloride, sodium bicarbonate, sodium chloride, trichloroacetic acid (≥99.0%), Fiske-Subbarow reducer reagent, ammonium molybdate, phosphorus standard solution, sodium deoxycholate (SDCh) and Triton™ X-100 were purchased from Sigma-Aldrich Chemie (Steinheim, Germany). Potassium dihydrogen phosphate, glucose, hydrogen peroxide (30%) and calcium chloride were obtained from Merck KGaA (Darmstadt, Germany). Propylene glycol (PG) was purchased from NMD -Norwegian Medical Depot AS (Oslo, Norway), Albunorm ® (human serum albumin, 200 mg/mL) from Octapharma AG (Lachen, Switzerland) and sucrose from VWR International bvba/sprl (Leuven, Belgium). Sulfuric acid was purchased from May and Baker LTD (Dagenham, UK). Cellophane membrane was obtained from Max Bringmann KG (Wendelstein, Germany) and pig ears from Nortura AS (Bardufoss, Norway).
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