Gfp rab7a

Manufactured by Addgene

Sourced in United States

GFP-Rab7A is a fluorescent protein-tagged version of the Rab7A protein. Rab7A is a small GTPase that plays a key role in late endosome and lysosome biogenesis and function. The GFP tag allows for visualization and tracking of the Rab7A protein within cells.

Automatically generated - may contain errors

Lab products found in correlation

In fusion technique, by Takara Bio (1 mentions) Mkate2 p2a apex2 tapp1 ph, by Addgene (1 mentions) Ptag bfp c h rab5a c myc, by Addgene (1 mentions) Lamp1 mgfp, by Addgene (1 mentions) Megfp n1, by Addgene (1 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions)

3 protocols using gfp rab7a

Fluorescent Biosensor Cloning Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To construct the mKate2-TAPP1-PH, BamHI-mKate2 and TAPP1-PH-Not1 were prepared by PCR with mKate2-P2A-APEX2-TAPP1-PH (Addgene number; #67662) as a template. These PCR products were inserted in pRK5 vector using In-Fusion technique (Takara). pTag-BFP-C-h-Rab5a-c-Myc (#79801), GFP-Rab7a (#61803), LAMP1-mGFP (#34831), and pEGFPN1-human Dynamin K44A (#22197) were obtained from Addgene. FRET-based RhoA biosensor is originally from Dr. Matsuda (Osaka University)^{39 (link)}. mCherry-tagged ARAP3 and ARAP3-R982A were generated by fusing red fluorescent protein mCherry into the N-terminal of the ARAP3 templates^{34 (link)}. To construct the EGFP-ARAP3-PH(X), each PH domain^{35 (link)} and EGFP sequences were prepared by PCR and inserted in pRK5 vector using In-Fusion technique. VN173-tagged TAPP1 was constructed by inserting PCR fragments of VN173 and TAPP1 PH domain in pRk5 vector. VC155-tagged ARAP3-PH(X) constructs were generated by Xba1/BamH1 replacement of EGFP in EGFP-ARAP3-PH(X) with a VC155 including Xba1/BamH1 site.

Lee H.N., Sim K.M., Kim H., Ju J., Pae A.N., Park J.B., Ryu H, & Seong J. (2019). Aβ modulates actin cytoskeleton via SHIP2-mediated phosphoinositide metabolism. Scientific Reports, 9, 15557.

+ Open protocol

+ Expand

Lentiviral Constructs for Mitochondrial Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

pLV-EF1α-Mito-DsRed was generated from VectorBuilder (Chicago, IL, USA) and pLV-EF1α-ito-DsRed expressing lentivirus was produced at Boston Children’s Hospital (BCH) Viral Core. GFP-Rab7A (#61803), mEGFP-N1 (#54767), mito-meGFP (#172481) were obtained from Addgene (Watertown, MA, USA).

Wu C.I., Vinton E.A., Pearse RV I.I., Heo K., Aylward A.J., Hsieh Y.C., Bi Y., Adeleye S., Fancher S., Duong D.M., Seyfried N.T., Schwarz T.L, & Young-Pearse T.L. (2022). APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer’s pathology in trisomy 21 neurons. Molecular psychiatry, 27(4), 1970-1989.

+ Open protocol

+ Expand

Generation of Rab GTPase Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Ruby3-Rab7A (Addgene plasmid #135651), Ruby3-Rab6A (Addgene plasmid #135653), and Ruby3-Rab10 cDNA constructs cloned into pBS vectors under the control of the weak promoter L30 were generated by the Montpellier Genomic Collection (MGC). The GFP-Rab7A (#61803) and GFP-Rab7A T22N (#28048) plasmids were obtained from Addgene. The siRNA-resistant GFP-Rab7A construct was generated using the forward primer 5′-AGTATTCGATGTGACTGCCCCCAACAC-3′ and the reverse primer 5′-AGTACACAGCAGTCTGCACCTCTGTAGAAG-3′. Site-directed mutagenesis was carried out using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions.

Deffieu M.S., Cesonyte I., Delalande F., Boncompain G., Dorobantu C., Song E., Lucansky V., Hirschler A., Cianferani S., Perez F., Carapito C, & Gaudin R. (2021). Rab7-harboring vesicles are carriers of the transferrin receptor through the biosynthetic secretory pathway. Science Advances, 7(2), eaba7803.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!