Immunohistochemistry (IHC) was applied to assess the protein levels of PLK4, Ki67, p-ERK and p-p38 in paraffin-embedded samples of CRC tissue according to previously described methods17 (link). An anti-PLK4 antibody was used at the concentration of 1:200 (Abcam, Cambridge, UK), and anti-Ki67, p-ERK, p-p38 antibody was used at the concentration of 1:500, 1:800, 1:500, respectively (Cell Signaling Technology, Danvers, USA). The percentage of PLK4-positive cells was evaluated from 0 to 3 (0, no positive cells; 1, < 30% positive cells; 2, 30-60% positive cells; and 3, 60%-100% positive cells). The PLK4 staining intensity was scored across a range of four grades (0, no positive staining; 1, weakly positive staining; 2, moderately positive staining; and 3, strongly positive staining). Finally, the IHC staining intensity and percentage scores were multiplied using a specific formula to obtain an intermediate score, which was then used to calculate the final IHC score.
Anti plk4 antibody
Manufactured by Abcam
Sourced in United Kingdom
The Anti-PLK4 antibody is a laboratory reagent used to detect the expression of the PLK4 (Polo-like kinase 4) protein in biological samples. PLK4 is a serine/threonine-protein kinase that plays a crucial role in the regulation of centrosome duplication and mitotic spindle formation. This antibody can be utilized in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the PLK4 protein in different cell types and tissues.
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2 protocols using anti plk4 antibody
1
Quantitative Immunohistochemical Analysis of CRC
2
Comprehensive Protein Expression Analysis
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The specific primary antibodies used in this study were anti-PLK4 antibody (Abcam, Cambridge, UK; cat. no. ab137398), rabbit anti-LIN28A antibody (cat. no. 8641; Cell Signaling Technology, Inc., Danvers, MA, USA), rabbit anti-Ki67 antibody (cat. no. ab15580; Abcam) and mouse anti-β-actin antibody (cat. no. sc47778; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-rabbit IgG or anti-mouse IgG (cat. nos. 7074 and 7076, respectively; Cell Signaling Technology, Inc.) at a 1:10,000. Cell protein extraction reagent was obtained from CWBioTec (cat. no. CW0889; Jiangsu Kangwei Century Biotechnology Co., Ltd., Beijing, China).
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