The mRNA was measured using real-time RT-PCR with Taqman one-step RT-PCR reagents and results were normalized to co-amplified GAPDH. The primers and probes are listed as following: ACSL3 (Hs00244853_m1), MBOAT2 (Hs01027245_m1), ELOVL5 (Hs01094711_m1), ELOVL7 (Hs00405151_m1), SC5D (Hs00999007_m1), NANS (Hs00219054_m1), TMPRSS2 (Hs01120965_m1), NKX3.1 (Hs00171834_m1) (purchased from Applied Biosystems); PSA: forward, 5′-GATGAAACAGGCTGTGCCG-3′, reverse, 5′-CCTCACAGCTACCCACTGCA-3′, probe, 5′-FAM-CAGGAACAAAAGCGTGATCTTGCTGGG-3′; AR-V7: forward: 5′-CGGAAATGTTATGAAGCAGGGATGA-3′, reverse, 5′-CTGGTCATTTTGAGATGCTTGCAAT-3′, probe, 5′-FAM-GGAGAAAAATTCCGGGT-3′. For immunoblotting, cells were lysed with RIPA buffer with protease inhibitors and anti-Ser240/244 phosphorylated S6 (Cell Signaling), anti-AR (Millipore), anti-AR-V7, anti-ELOVL7, anti-β-actin, anti-GAPDH (Abcam), or anti-β-tublin (Upstate) antibodies were used. Gels shown are representative of at least 3 independent experiments.
Mboat2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MBOAT2 is a laboratory instrument designed for the analysis and detection of specific molecular targets. It utilizes a specialized technique to facilitate the identification and characterization of these targets within a sample. The MBOAT2 is a versatile tool intended for use in various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ar, by Merck Group (2 mentions)
Elovl5, by Thermo Fisher Scientific (2 mentions)
Elovl7, by Thermo Fisher Scientific (2 mentions)
Tmprss2, by Thermo Fisher Scientific (2 mentions)
Anti elovl7, by Abcam (2 mentions)
Acsl3, by Thermo Fisher Scientific (2 mentions)
Anti ar v7, by Abcam (2 mentions)
Anti β actin, by Abcam (2 mentions)
Anti gapdh, by Abcam (2 mentions)
2 protocols using mboat2
1
Quantitative RT-PCR and Immunoblotting Protocol
2
Quantification of mRNA Expression Using RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mRNA was measured using real-time RT-PCR with Taqman one-step RT-PCR reagents and results were normalized to co-amplified GAPDH. The primers and probes are listed as following: ACSL3 (Hs00244853_m1), MBOAT2 (Hs01027245_m1), ELOVL5 (Hs01094711_m1), ELOVL7 (Hs00405151_m1), SC5D (Hs00999007_m1), NANS (Hs00219054_m1), TMPRSS2 (Hs01120965_m1), NKX3.1 (Hs00171834_m1) (purchased from Applied Biosystems, Foster City, CA, USA); PSA: forward, 5′-GATGAAACAGGCTGTGCCG-3′, reverse, 5′-CCTCACAGCTACCCACTGCA-3′, probe, 5′-FAM-CAGGAACAAAAGCGTGATCTTGCTGGG-3′ AR-V7: forward: 5′-CGGAAATGTTATGAAGCAGGGATGA-3′, reverse, 5′-CTGGTCATTTTGAGATGCTTGCAAT-3′, probe, 5′-FAM-GGAGAAAAATTCCGGGT-3′. For immunoblotting, cells were lysed with RIPA buffer with protease inhibitors and anti-Ser240/244 phosphorylated S6 (Cell Signaling, Danvers, MA, USA), anti-AR (Millipore, Billerica, MA, USA), anti-AR-V7, anti-ELOVL7, anti-β-actin, anti-GAPDH (Abcam, Cambridge, MA, USA), or anti-β-tubulin (Upstate, Billerica, MA, USA) antibodies were used. Gels shown are representative of at least 3 independent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!