Bt549

Recombinant human IL1B (200-01B) and IL-1R antagonist (IL1-RA, 200-01R) were purchased from Peprotech (Rocky Hill, NJ). p38 MAPK (8690), phospho-p38 MAPK (Thr180/Tyr182; 4511), p42/44 MAPK (9107S), phospho-p42/44 MAPK (Thr202/Tyr204; 9101) antibodies were purchased from Cell Signaling Technology (Beverly, MA). MAPK inhibitors SP600125, SB202190 and SB203580 were purchased from Sigma Aldrich (St Louis, MO), U0126 and BAY869766 were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA).
The human breast cancer lines: MDA-MB-231, MDA-MB-436, BT549, SKBR3, ZR75-1, HCC1954 were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA), 2 mM L-glutamine, and 50 μg/mL gentamicin (Life Technologies, Carlsbad, CA). THP-1 monocyte cells were cultured in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, and 1% antibiotic/antimycotic solution (15240062, Life Technologies). All cell lines were recently acquired from the ATCC (Manassas, VA). Cell lines were incubated in a humidified atmosphere of 5% CO₂ at 37 °C.

MCF-7 (HER2 positive), MDAMB-231 (TNBC), BT-549 (TNBC), and SK-BR-3 (HER-2 positive) breast cancer cell lines were obtained from ATCC (Manassas, VA, USA). MCF-7, and MDAMB-231 were maintained in DMEM medium (Corning, NY, USA) supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA) and penicillin-streptomycin (Corning, NY, USA) at 5% CO₂/37 °C. BT-549 was maintained in RPMI-1640 medium supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA), 1% sodium pyruvate (Corning, NY, USA), 1% penicillin-streptomycin (Corning, NY, USA), 0.023 U/mL of Gibco™ Insulin, human recombinant zinc solution (Fisher Scientific, Hampton, NH, USA)). SK-BR-3 was maintained in Hyclone McCoy’s 5A Medium (GE Health care Life Sciences (Marlborough, MA, USA)) supplemented with 10% FBS (Atlanta Biologicals, Minneapolis, MN, USA) and 1% penicillin-streptomycin (Corning, NY, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), crystal violet dye, and 16% paraformaldehyde (PFA) solution were purchased from Fisher Scientific (Hampton, NH, USA). All molecular biology kits and supplies were purchased from other commercial vendors which are listed at appropriate places throughout the manuscript.