Hgf pcs 201 018

Cell culture conditions were 37 °C, 5 % CO₂, and 21 % O₂. The immortalised plexiform ameloblastoma cell line, AM-1 was provided by Professor Harada [17 (link)]. Keratinocyte serum free medium 1X (KSFM) supplemented with KSFM supplements (bovine pituitary extract (BPE) and epidermal growth factor (EGF), human recombinant) was used to culture the AM-1 cells. Primary gingival fibroblasts, Human, Adult (HGF) (PCS-201-018™) were purchased from ATCC and cultured in Dulbecco's modified Eagle medium (DMEM). Primary human osteoblasts (hOB) from Promocell® (Heidelberg, Germany) were cultured in Promocell® osteoblast growth medium with supplement mix. All media types contained 10 % foetal bovine serum (FBS), 100 units/mL penicillin, and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific, Loughborough, UK).

The following cell lines were used in this study: human gingival fibroblasts—HGF (PCS-201-018™) and squamous cell carcinoma cell line—SCC-4 (CRL-1624™), both purchased from ATCC. The acquired cells came as frozen vials and were kept in liquid nitrogen. The cell lines were cultured in their specific growth medium: HGF in Fibroblast Basal Medium which was completed with the Fibroblast Growth Kit-Low Serum; 10% FCS and 1% antibiotic mixture penicillin–streptomycin–amphotericin B Solution, 0.5 mL (Final concentration penicillin: 10 Units/mL, streptomycin: 10 µg/mL, and amphotericin B: 25 ng/mL); and SCC-4 in DMEM: F12 Medium supplemented with 400 ng/mL hydrocortisone 21-hemisuccinate sodium salt, 10% FCS, and 1% penicillin (100 U/mL)–streptomycin (100 μg/mL). The cells were cultivated under standard culture conditions at 37 °C and 5% CO2 in a humidified atmosphere.