1H NMR spectra were recorded on a Bruker AV-500NMR spectrometer in deuterated DMSO. Dynamic light scattering (DLS) was detected on a Wyatt QELS instrument with a vertically polarized He-Ne laser (DAWN EOS, Wyatt Technology Co., Santa Barbara, California, USA). The ultraviolet (UV) absorption spectrum was acquired on a UV-2401PC spectrophotometer (Shimadzu, Japan). Transmission electron microscopy (TEM) measurements were made on a JEOL JEM-1011 transmission electron microscope (Tokyo, Japan) with an accelerating voltage of 100 kV. Flow cytometry analysis (FCA) was conducted on a Guava EasyCyte™ 12 Flow Cytometer (Millipore, Billerica, MA, USA) and confocal laser scanning microscopy (CLSM) was performed on a CLSM 780 (Carl Zeiss). High-performance liquid chromatography (HPLC) was performed using a binary HPLC pump with a C18 column (250 mm × 4.6 mm; made in Ireland).
Guava easycyte 12 flow cytometer
Manufactured by Merck Group
Sourced in United States
The Guava EasyCyte™ 12 Flow Cytometer is a compact, bench-top instrument designed for multi-parameter cell analysis. It utilizes flow cytometry technology to enable the simultaneous measurement of multiple characteristics of individual cells within a heterogeneous population.
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Lab products found in correlation
2 protocols using guava easycyte 12 flow cytometer
1
Multimodal Characterization of Nanomaterials
2
Phenotypic Profiling of Cell Populations
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Freshly harvested cells were washed in FACS buffer (pH 7.4 PBS with 1 mM MgCl2, 0.1 mM CaCl2, 1% FBS and 0.02% sodium azide) and aliquoted into 1×106 fractions for staining. CD24-FITC and CD44-APC antibodies (BD Biosciences) were added according to the manufacturer’s recommended dilution and the samples were incubated on ice with shaking for 30 min. After two washes, samples were resuspended in 500 μl of FACS buffer and analyzed on a Millipore Guava easyCyte 12 flow cytometer. HER2 was stained using 1 μg of trastuzumab per sample followed by washing and incubation with 1 μg of Alexa 647 conjugated anti-human secondary antibody.
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