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Ethylenediaminetetraacetic acid disodium salt dehydrate

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Ethylenediaminetetraacetic acid disodium salt dehydrate is a chemical compound used as a laboratory reagent. It is a chelating agent that binds metal ions, and is commonly used in various analytical and research applications.

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Lab products found in correlation

Trizma base, by Merck Group (4 mentions) Sodium dodecyl sulfate (sds), by Merck Group (4 mentions) Bovine serum albumin (bsa), by Merck Group (4 mentions) Igepal ca 630, by Merck Group (3 mentions) N ethylmaleimide, by Merck Group (3 mentions) Tween 20, by Merck Group (3 mentions) Iodoacetamide, by Merck Group (3 mentions) Triton x 100, by Merck Group (3 mentions) Dl dithiothreitol, by Merck Group (3 mentions) Complete protease inhibitor cocktail, by Roche (3 mentions) Phosphatase inhibitor cocktail, by Roche (3 mentions) Chitosan, by Merck Group (2 mentions) Magnesium chloride hexahydrate, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Cacodylic acid, by Merck Group (1 mentions) Lead acetate, by Merck Group (1 mentions) Ammonium sulfamate, by Merck Group (1 mentions)

Close spelling variants of this product

Ethylenediaminetetraacetic acid (561 citations) Ethylenediaminetetraacetic acid disodium salt (17 citations) Ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) (7 citations)

14 protocols using ethylenediaminetetraacetic acid disodium salt dehydrate

1

Oxidative Stress Measurement Protocol

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Trizma base, cacodylic acid, diethylenetriaminepentaacetic acid (DTPA), pyrogallol, ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA), glutathione reductase (from Baker’s yeast; GSH-R), L-glutathione reduced (GSH), β-nicotinamide adenine dinucleotide hydrate (NADPH), pipes, potassium ferricyanide, ammonium sulfamate, lead acetate, trichloroacetic acid (TCA), 2,4- dinitrophenylhydrazine (DNPH), guanidine hydrochloride, and serum albumin from bovine were purchased from Sigma-Aldrich Co. LLC. (USA). The 2-thiobarbituric acid was purchased from Alfa Aesar (USA). Ethanol, ethyl acetate, and chloroform were obtained from J. T. Baker (USA). Deionized water was made with a Milli-Q Water Purification Equipment (Millipore SAS, France).
Kang S.M., Kang G., Seong P., Park B, & Cho S. (2014). Effect of Packaging Method on the Lipid Oxidation, Protein Oxidation, and Color in Aged Top Round from Hanwoo (Korean Native Cattle) during Refrigerated Storage. Korean Journal for Food Science of Animal Resources, 34(3), 273-279.
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2

Preparation of Functionalized Styrene Particles

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Styrene, d-glucose, d-galactose, d-fructose, d-(+)-fucose, phenylmethylsulfonyl fluoride (PMSF), sodium dodecyl sulfate (SDS), Tris, KCl, ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA) and bovine serum albumin (BSA, essentially fatty acid free) were purchased from Sigma-Aldrich. 1-Propanol, sodium phosphate monobasic dihydrate and sodium dihydrogen phosphate monohydrate were purchased from J. T. Baker Inc. These chemicals were used as received. Glutaraldehyde (50 wt% in water) was purchased from Sigma-Aldrich and diluted to a 2.5 wt% aqueous stock solution prior to use. Ultrapure water with a resistivity > 18.2 MΩ cm–1 was obtained from an ultrapure water system (Barnstead). Glass slides (25 mm × 75 mm × 1 mm) were purchased from Fisher Scientific.
Cai Z., Luck L.A., Punihaole D., Madura J.D, & Asher S.A. (2016). Photonic crystal protein hydrogel sensor materials enabled by conformationally induced volume phase transition †Electronic supplementary information (ESI) available: Mechanism of the crosslinking reaction, protein sequence of GGBP, UVRR spectra, UV-Vis reflection spectra of 2-D PC-GGBP sensor at different glucose concentrations and additional information of crystal structures of GGBP. See DOI: 10.1039/c6sc00682e. Chemical Science, 7(7), 4557-4562.
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3

Biochemical Reagents for Cell Signaling

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DL-Dithiothreitol (DTT, D0632), N-Ethylmaleimide (NEM, E1271), Iodoacetamide (I1149), IGEPAL CA-630 (NP40, I3021), Triton X-100 (T9284), bovine serum albumin (BSA, A7906), Sodium dodecyl sulfate (SDS, L3771), Tween-20 (P9416), Ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA, E4884) and Trizma base (Tris, 93349), Sodium butyrate (NaBu, B5887) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 4174) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Complete protease inhibitor cocktail (04693116001), phosphatase inhibitor cocktail (04906837001) and alkaline phosphatase (11097075001) were purchased from Roche Diagnostic (Mannheim, Germany). Ciprofloxacin (17850) was purchased from Fluka (Buchs, Switzerland). Poly(I:C) (LMW)/LyoVec (tlrl-picwlv) was purchased from Invivogen (Toulouse, France).
Gupta S., Ylä-Anttila P., Callegari S., Tsai M.H., Delecluse H.J, & Masucci M.G. (2018). Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathogens, 14(1), e1006852.
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4

Formulation and Characterization of Amphotericin B and Levofloxacin Nanoparticles

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Amphotericin B (AMB) was purchased from Cayman Chemical Company (purity specification ≥ 95%), Ann Arbor, MI, USA. Levofloxacin hydrochloride (LVX) was obtained from Zhejiang Jingxin Pharmaceuticals (Xinchang, China). Plasdone™ K-29/32 (poly(vinylpyrrolidone), PVP) (MW 58 kDa) was donated by Ashland (Kidderminster, UK). Chitosan (low molecular weight), ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA-Na2), dodecyl sodium sulphate (SLS) and HPLC-grade methanol were also supplied by Sigma-Aldrich (Poole, Dorset, UK). Dimethyl sulfoxide (DMSO) was provided by VWR International Limited, Leicestershire, UK. Phosphate buffered saline (PBS) pH 7.4 tablets were acquired from Oxoid Limited, Hampshire, UK. All other chemical reagents were of analytical grade and purchased from Sigma-Aldrich (Dorset, UK) or Fisher Scientific (Leicestershire, UK).
Peng K., Li M., Himawan A., Domínguez-Robles J., Vora L.K., Duncan R., Dai X., Zhang C., Zhao L., Li L., Larrañeta E, & Donnelly R.F. (2022). Amphotericin B- and Levofloxacin-Loaded Chitosan Films for Potential Use in Antimicrobial Wound Dressings: Analytical Method Development and Its Application. Pharmaceutics, 14(11), 2497.
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5

Hexoprenaline Sulphate Injection Formulation

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The reference working standard of hexoprenaline sulphate (purity of 90.3%) was acquired from BOC Sciences (Shirley, NY, USA). The samples of hexoprenaline sulphate as injectable dosage form (10 µg/2 mL) (Gynipral®) were acquired from Takeda Austria GmbH (Linz, Austria). Sodium chloride, sodium disulfite from Merck (Darmstadt, Germany) and ethylenediaminetetraacetic acid disodium salt dehydrate from Sigma-Aldrich (St. Louis, MO, USA) were used as excipients in the used hexoprenaline product (Gynipral®), as per the patient information leaflet (PIL) [48 ].
Afonso Urich J.A., Marko V., Boehm K., Lara García R.A., Jeremic D, & Paudel A. (2021). Development and Validation of a Stability-Indicating UPLC Method for the Determination of Hexoprenaline in Injectable Dosage Form Using AQbD Principles. Molecules, 26(21), 6597.
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6

Hybrid Biomaterial for Cell Adhesion

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Poly(ethylene imine) solution (Mw =
750 000 g/mol), chitosan (medium molecular
weight), heparin sodium salt (from porcine intestinal mucosa), phenylmethanesulfonyl
fluoride, sodium bicarbonate, ethylenediaminetetraacetic acid disodium
salt dehydrate, rhodamine B isothiocyanate, sodium chloride, ammonium
fluoride (NH4F), and fibrinogen from human plasma were
obtained from Sigma-Aldrich. Silica spheres with a diameter of 5 μm
were obtained from Microparticles GmbH, Germany. Protein BCA protein
assay kit, Dulbecco’s modified Eagle’s medium (DMEM,
high glucose), PBS (1 ×, pH 7.4), cell membrane marker (Wheat
germ agglutinin, Alexa Fluor 488 conjugate), thrombin, and Alexa Fluor
488-conjugated fibrinogen were purchased from ThermoFisher Scientific.
Mini-PROTEAN@ TGX stain-free gels, 4 × Laemmli protein sample
buffer, and Precision Plus Protein All Blue Standards were obtained
from Bio-Rad. Ultrapure water (Millipore) of 18.2 MΩ·cm
was used for all experiments. All chemicals were used as received.
Shao J., Abdelghani M., Shen G., Cao S., Williams D.S, & van Hest J.C. (2018). Erythrocyte Membrane Modified Janus Polymeric Motors for Thrombus Therapy. ACS Nano, 12(5), 4877-4885.
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7

Protein Extraction and Inhibitor Cocktail

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DL-dithiothreitol (DTT, D0632), N-ethylmaleimide (NEM, E1271), iodoacetamide (I1149), IGEPAL CA-630 (NP40, I3021), Triton X-100 (T9284), bovine serum albumin (BSA, A7906), sodium dodecyl sulfate (SDS, L3771), Tween-20 (P9416), ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA, E4884), Trizma base (Tris, 93349) and ciprofloxacin (I7850) were purchased from Sigma–Aldrich. Complete protease inhibitor cocktail (04693116001) and phosphatase inhibitor cocktail (04906837001) were purchased from Roche Diagnostic.
Ylä-Anttila P, & Masucci M.G. (2021). Inhibition of selective autophagy by members of the herpesvirus ubiquitin-deconjugase family. Biochemical Journal, 478(12), 2297-2308.
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8

Functionalization of Oxidized Multi-Walled Carbon Nanotubes

Cited 1 time
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Short oxidized MWCNTs with 0.5–2 μm in length and in three diameters (<8 nm; 8–15 nm; 20–30 nm) were purchased from Nanostructured and Amorphous Materials. Distearoyl-sn-glycero-3- phosphoethanolamine conjugated to polyethylene glycol5000 terminated with a methoxy group (DSPE-PEG), maleimide (DSPE-PEG-Mal), and fluorescein isothiocyanate (DSPE-PEG-FITC) were purchased from Nanocs, Inc. N-Succinimidyl S-acetylthioacetate (SATA) and hydroxylamine hydrochloride were purchase from Thermo Scientific. Ethylenediaminetetraacetic acid disodium salt dehydrate, sodium hydroxide solution, penicillin and streptomycin were obtained from Sigma-Aldrich. An anti-Pgp monoclonal antibody (15D3) was produced in house using a method described previously.38 (link) Water used in all experiments was purified using a Milli-Q water purification system (Millipore, S.A.S, B.P).
Suo X., Eldridge B.N., Zhang H., Mao C., Min Y., Sun Y., Singh R, & Ming X. (2018). P-glycoprotein-Targeted Photothermal Therapy of Drug-Resistant Cancer Cells using Antibody-Conjugated Carbon Nanotubes. ACS applied materials & interfaces, 10(39), 33464-33473.
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9

Synthesis of Photocatalytic Nanocomposites

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Zn(NO3)2.6H2O, Na2WO4.2H2O were used as precursors of zinc and tungstate oxide, respectively. Manganese (III) phthalocyanine, 1,3-propanesultone (>99%), Dimethylformamide (DMF) (>99%), Dichloro methane (CH2Cl2) (>99%), bisphenol A (2,2-bis (4-hydroxyphenyl) propane salt (>99%), NaNO3, Na2CO3, NaCl, AlCl3, CaCl2, KCl, and sodium humic acid, all of high purity (>99%) and p-benzoquinone (BQ) (≥98%), isopropyl alcohol (IPA) (≥99%), Ethylenediamine tetraacetic acid disodium salt dehydrate (EDTA) (≥99%), hydrogen peroxide (H2O2) (30% v/v), hydrochloric acid (HCl) (37% wt./v), sodium hydroxide (NaOH) (≥99%) were used as purchased from Sigma Aldrich (Darmstadt, Germany) without any further treatment. Mili-Q water has been used for the entire photocatalytic experimental studies.
Anucha C.B., Altin I., Biyiklioglu Z., Bacaksiz E., Polat I, & Stathopoulos V.N. (2020). Synthesis, Characterization, and Photocatalytic Evaluation of Manganese (III) Phthalocyanine Sensitized ZnWO4 (ZnWO4MnPc) for Bisphenol A Degradation under UV Irradiation. Nanomaterials, 10(11), 2139.
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10

Isolation and Characterization of Biomolecules

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Sea salt (Red Sea Salt) was purchased from Red Sea Fish Pharm. Ltd. (Eilat, Israel). Sodium nitrate was obtained from Daejung Chemical & Metals (Siheung, Republic of Korea). Organic solvents, including absolute ethanol, acetonitrile (ACN), and methanol, were obtained from Fisher Scientific (Pittsburgh, PA, USA). Bovine serum albumin was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Ethylenediaminetetraacetic acid disodium salt dehydrate (Na2EDTA), magnesium chloride hexahydrate (MgCl2·6H2O), manganese(II) chloride tetrahydrate (MnCl2·4H2O), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES), potassium chloride (KCl), sorbitol, and 1,4-dithiothreitol (DTT) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The FX standard was purchased from Sigma-Aldrich.
Truong T.Q., Park Y.J., Winarto J., Huynh P.K., Moon J., Choi Y.B., Song D.G., Koo S.Y, & Kim S.M. (2024). Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation. Marine Drugs, 22(2), 93.
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