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Chicken anti rabbit igg h l alexa fluor 647

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Chicken anti-rabbit IgG (H+L) Alexa Fluor 647 is a secondary antibody that binds to rabbit primary antibodies. It is labeled with the Alexa Fluor 647 fluorescent dye, which emits in the red/far-red region of the spectrum when excited.

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Lab products found in correlation

Hrp conjugated anti mouse and anti rabbit igg, by GE Healthcare (2 mentions) Anti na k atpase, by Abcam (1 mentions) Anti v5, by Thermo Fisher Scientific (1 mentions) Anti flag m2 hrp, by Merck Group (1 mentions)

2 protocols using chicken anti rabbit igg h l alexa fluor 647

1

Immunofluorescence and Western Blotting Protocols

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Primary antibodies were as follows: rabbit polyclonal anti-β4 (32 (link)), mouse monoclonal anti-V5 (Life Technologies, R960-25), and mouse monoclonal anti-Na+/K+ ATPase (Abcam, ab7671). Secondary antibodies were as follows: chicken anti-rabbit IgG (H+L) Alexa Fluor 647 (Life Technologies, A21443), which was used for immunofluorescence, and HRP-conjugated anti-mouse and anti-rabbit IgG (GE Healthcare, NA931 and NA934, respectively), which were used for Western blotting.
Shimizu H., Tosaki A., Ohsawa N., Ishizuka-Katsura Y., Shoji S., Miyazaki H., Oyama F., Terada T., Shirouzu M., Sekine S.I., Nukina N, & Yokoyama S. (2017). Parallel homodimer structures of the extracellular domains of the voltage-gated sodium channel β4 subunit explain its role in cell–cell adhesion. The Journal of Biological Chemistry, 292(32), 13428-13440.
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2

Antibody Characterization for Membrane Proteins

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Primary antibodies were as follows: rabbit polyclonal anti-β1 and anti-β433 (link); rabbit polyclonal anti-β1ex (Abcam ab107370); mouse monoclonal anti-Na+/K+ ATPase (Abcam, ab7671); mouse monoclonal anti-V5-HRP (Life Technologies, R961-25); polyclonal anti-V5 (Funakoshi, GeneTex, GTX117997); and anti-Flag M2-HRP (SIGMA, A8592). The secondary antibodies included chicken anti-rabbit IgG (H+L)-Alexa Fluor 647 (Life Technologies, A21443), which was used for immunofluorescence, and HRP-conjugated anti-mouse and anti-rabbit IgG (GE Healthcare Bio-Sciences, NA931 and NA934, respectively), which were used for western blotting.
Shimizu H., Miyazaki H., Ohsawa N., Shoji S., Ishizuka-Katsura Y., Tosaki A., Oyama F., Terada T., Sakamoto K., Shirouzu M., Sekine S.I., Nukina N, & Yokoyama S. (2016). Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits. Scientific Reports, 6, 26618.
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