Immunohistochemistry was performed on 3 μm sections derived from formalinfixed paraffin-embedded (FFPE) samples. Samples were immunhistochemically stained by using the Ventana Benchmark system (Roche, Basel, Switzerland) according to the manufacterer´s protocol. Antibodies against cytokeratin 5/6 (CK5/6; 1:50, Dako; Hamburg, Germany), cytokertain 7 (CK7; 1:150, ZytoVision, Bremerhaven, Germany), cytokeratin 14 (CK14; 1:300, DC Systems, Hamburg, Germany), p53 (1:200; Dako, Hamburg, Germany), S100 (1:6000, Dako, Hamburg, Germany), estrogen receptor and progesteron receptor (each 1:20, DC Systems, Hamburg, Germany), Her2/neu (1:300, Dako, Hamburg, Germany), mammaglobin (1:100, Menarini Diagnsotics, Berlin, Germany), muc 4 (1:300, Santa Cruz, Heidelberg, Germany), prostate-specific antigen (PSA; 1:3000, Dako, Hamburg, Germany), androgen receptor (AR; 1:1; CellMarque, Darmstadt, Germany) and Ki67/MIB-1 (1:50, Dako, Hamburg, Germany) were applied.
Her2 neu
Manufactured by Agilent Technologies
Sourced in Germany
Her2/neu is a laboratory equipment product designed for the detection and analysis of the HER2/neu protein. It is a key component in the assessment of breast cancer prognosis and treatment selection. The product provides reliable and accurate measurement of HER2/neu expression levels, which is essential for guiding clinical decisions.
Automatically generated - may contain errors
3 protocols using her2 neu
1
Comprehensive Immunohistochemical Profiling
2
IHC-Based HER2 Expression Scoring
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HER2 expression was examined in the tumor tissue by IHC19 (link),49 (link). Briefly, 4-μm sections were deparaffinized, rehydrated, and treated with 3% hydrogen peroxide-methanol at room temperature for 5 min and then heated in a water bath at 98 °C for 40 min in the target retrieval solution (pH 9.0; Dako, Glostrup, Denmark). The sections were blocked with 8% skim milk in Tris-buffered saline (TBS) at 37 °C for 40 min and then incubated with a rabbit polyclonal anti-human c-erbB-2 oncoprotein (HER2/neu, 1:100 dilution; Dako) at 37 °C for 40 min. The sections were incubated with the EnVision polymer reagent for rabbit IgG (Dako) at 37 °C for 60 min. Then, the reaction products were visualized using 3,3-diaminobenzidine. HER2 immunoreactivity was quantified based on a previous study19 (link). Samples with HER2 IHC scores of 0 and 1 were classified as negative, and those with scores of 2 and 3 were classified as positive.
3
Immunohistochemical Analysis of CK5, CK20, and HER2
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Immunohistochemical stainings were carried out on a BenchMark Ultra (Ventana, Tucson, Arizona) using clinical-grade (CLIA) antibodies according to the manufacturer's instructions. The antibodies were diluted as follows: CK5 1:50 (Diagnostic BioSystems), CK20 1:50 (Dako) and Her2/neu 1:1000 (Dako). All stainings were reviewed by two pathologists (ME, AH).
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