Control mirna inhibitor

Cell line SKNO-1 was plated at a concentration of 10,000 cells per well in triplicate for each condition in a 96-well plate 24 h after electroporation with the miR-130a inhibitor or the control miRNA inhibitor (Qiagen). Etoposide (5 μM, Sigma, München, Germany) or the same volume of DMSO (Sigma) (mock treatment) was added to each well. After 24 h, MTT (5 μg/mL, Sigma) was added to each well. After a 3-h incubation in 37 °C, reactions were stopped by adding DMSO/isopropanol (2:1). Absorbance was determined at 570 nm by an ELISA reader (Thermo Electron, Vantaa, Finland). For apoptosis, caspase 3/7 activity was detected using the ApoONE Homogeneous Caspase 3/7 Assay (Promega) following the manufacturer’s instruction. Fluorescence was measured by a spectrofluorometer instrument (Perkin Elmer, Waltham, MA, USA). The cell viability and apoptosis assays were performed in triplicate.

The miR-105-5p inhibitor and control miRNA inhibitor were purchased from QIAGEN. The miR-105-5p inhibitor and control miRNA inhibitor were transferred to iMSC-sEVs by using the Exo-Fect siRNA/miRNA Transfection Reagent Kit (SBI), according to the manufacturer’s instructions.