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Anti cd8α

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Anti‐CD8α is a laboratory reagent that can be used to detect the presence of the CD8α molecule, which is expressed on the surface of certain types of T cells and natural killer cells. It is a monoclonal antibody that specifically binds to the CD8α subunit.

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Lab products found in correlation

Envision system, by Agilent Technologies (1 mentions) Anti cd4, by Cell Signaling Technology (1 mentions) Anti foxp3, by Cell Signaling Technology (1 mentions) Opal fluorescent ihc kit, by PerkinElmer (1 mentions) Ab182422, by Abcam (1 mentions) Ab28364, by Abcam (1 mentions) Ab6640, by Abcam (1 mentions) Fv1000d, by Olympus (1 mentions) Ab53066, by Abcam (1 mentions) Sc 651, by Santa Cruz Biotechnology (1 mentions) Gtx16667, by GeneTex (1 mentions) Sc 152, by Santa Cruz Biotechnology (1 mentions) Ab5694, by Abcam (1 mentions) Biotinylated secondary antibody, by Jackson ImmunoResearch (1 mentions) 3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions) Histogel, by Thermo Fisher Scientific (1 mentions) α sma, by Cell Signaling Technology (1 mentions) 3 3 diaminobenzidine (dab), by Cell Signaling Technology (1 mentions) Bloxall blocking solution, by Vector Laboratories (1 mentions)

Spelling variants of this product

Anti-mouse CD8α (3 citations) Rabbit anti-mouse CD8α (D4W2Z) (2 citations)

4 protocols using anti cd8α

1

Comprehensive Immunohistochemical Analysis of Tumor Microenvironment

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At sacrifice, the livers containing tumours of each mouse were sampled, fixed in zinc‐formalin and sliced to 4‐µm sections. Immunohistochemistry was performed using the EnvisionTM + system (DAKO), and immunofluorescence staining was performed using the Opal Fluorescent IHC Kit (PerkinElmer) according to the manufacturer's instructions. The MI‐77 MW rapid processor (Azumaya) was used for some reactions in the process. Immunohistochemistry was performed using the following antibodies: anti‐Ki‐67 (GTX16667, GeneTex); anti‐CD31 (ab28364, Abcam); anti‐αSMA (ab5694, Abcam); anti‐FAP (ab53066, Abcam); anti‐CD4 (#25229, Cell Signaling); anti‐CD8α (#98941, Cell Signaling); and anti‐FOXP3 (#12653, Cell Signaling). Immunofluorescence staining was performed utilizing the following antibodies: anti‐F4/80 (ab6640, Abcam); anti‐NOS2 (sc‐651, Santa Cruz); anti‐CD163 (ab182422, Abcam); and anti‐VEGF‐A (sc‐152, Santa Cruz). A confocal microscope (FV1000D, Olympus) was used for fluorescence observation. For all the studies, randomly selected fields (100 × magnification) for every large metastasis (ie those> 1 mm2) were analysed using WinROOF version 3.6 (Mitani).
Masaki S., Hashimoto Y., Kunisho S., Kimoto A, & Kitadai Y. (2020). Fatty change of the liver microenvironment influences the metastatic potential of colorectal cancer. International Journal of Experimental Pathology, 101(5), 162-170.
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2

Quantifying CD8+ T Cells in MC38 Xenograft Tumors

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The MC38 xenograft tumors were harvested and formalin-fixed. The samples were embedded, sectioned and IHC staining was performed at The Wistar Institute Histotechnology Facility. Antigen retrieval was performed by steaming the slides in citrate buffer (pH 6.0) for 5 min. After washing with PBS, the sections were blocked and incubated with an anti-CD8α (1:200; Cell Signaling) antibody overnight at 4°C, followed by incubation with a biotinylated secondary antibody (1:200; Jackson Immuno Research) for 30 min. Detection was performed using DAB (Fisher Scientific). The slides were scanned in iHisto Inc. and visualized using QuPath-0.2.3 software. The CD8+ T cells that stained with strong membranous positivity were enumerated in five separate areas at 20× magnification in a blinded fashion.
Zhang W., Zhong W., Wang B., Yang J., Yang J., Yu Z., Qin Z., Shi A., Xu W., Zheng C., Schuchter L.M., Karakousis G.C., Mitchell T.C., Amaravadi R., Herlyn M., Dong H., Gimotty P.A., Daaboul G., Xu X, & Guo W. (2022). ICAM-1-mediated Adhesion is a Prerequisite for Exosome-induced T Cell Suppression. Developmental cell, 57(3), 329-343.e7.
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3

Tumor Organoid Immunohistochemistry Protocol

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Tumor organoids were embedded in 10% Histogel (Fisher scientific, 22‐110‐678) following the protocol from the product and then embedded in paraffin blocks. Immunohistochemistry (IHC) staining for tumors and organoid samples was performed using the protocol mentioned.[65] The primary antibodies used in this experiment include anti‐α‐Smooth Muscle Actin (α‐SMA) (Cell Signaling Technology, clone: D4K9N), anti‐glial fibrillary acidic protein (GFAP) (Cell Signaling Technology, Clone GA5, 3670), and anti‐CD8α (Cell Signaling Technology, clone: D4W2Z).
Zhou Z., Van der Jeught K., Li Y., Sharma S., Yu T., Moulana I., Liu S., Wan J., Territo P.R., Opyrchal M., Zhang X., Wan G, & Lu X. (2023). A T Cell‐Engaging Tumor Organoid Platform for Pancreatic Cancer Immunotherapy. Advanced Science, 10(23), 2300548.
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4

Immunohistochemical Analysis of CD3+ and CD8+ T Cells

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Ileal samples from all mice of each group were fixed with 4% paraformaldehyde and then embedded in paraffin. Four micrometer-thick sections were incubated with citrate 1X antigen repair solution (Skytec) at 95°C for 20 min after dewaxing and hydration. Then endogenous peroxidase was blocked by Bloxall blocking solution (Vector) for 10 min at RT. Nonspecific antigens were blocked with 5% goat serum for 30 min. The sections were exposed to primary anti-CD3γ (Abcam) and anti -CD8α (Cell Signaling Technology) Abs overnight at 4°C. After washing with Tris-buffered saline solution containing 0.05% Tween, sections were incubated for 30 min with the detection kit “Immpress peroxidase Polymer anti rabbit IgG” (Vector). Negative controls were incubated with irrelevant serum. The staining was revealed using the peroxidase substrate, DAB (Cell Signaling Technology). Hematoxylin counterstain was performed before mounting the slides in an aqueous medium. Slides were analyzed using a microscopy (Leica).
Hariss F., Delbeke M., Guyot K., Zarnitzky P., Ezzedine M., Certad G, & Meresse B. (2023). Cytotoxic innate intraepithelial lymphocytes control early stages of Cryptosporidium infection. Frontiers in Immunology, 14, 1229406.
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