L nmma

Polyclonal rabbit anti-PY⁷⁰⁵-STAT3 (#9145), anti-STAT3 (#4904) and anti-α/β-tubulin (#2148) were purchased from Cell Signaling Technology. For western blotting, the antibodies were probed using secondary donkey anti-rabbit antibodies, conjugated with horseradish peroxidase (Jackson Immunoresearch, #711-035-152). For immunofluorescence, the antibodies were probed using secondary donkey anti-rabbit antibodies conjugated to Rhodamine Red X (Jackson Immunoresearch, #711-295-152). Cell nuclei were fluorescently labelled using DAPI (Sigma-Aldrich, #D9564) or Syto 60 (Life Technologies, #S11342). Purified human IL-10 (#130-093-947), IL-6 (#130-095-365) and pure grade neutralizing anti-IL-6 (#130-096-093) and anti-IL-10 (#130-096-041) antibodies were purchased from Miltenyi Biotech. Nitric oxide synthase inhibitor L-NMMA (#0771, Tocris) was suspended in desionized water at the concentration of 50 mM.

Lipopolysaccharide from E. coli was purchased from Sigma (0127:B8). Fura-2 and Fluo-4 were purchased from Invitrogen. L-NAME, cobinamide, NAC and GSH were purchased from Sigma. PTIO and L-NMMA were purchased from Tocris Bioscience (Bristol, UK). MCI-186 was purchased from Calbiochem (San Diego, CA). Human TGF-β1 and TGF-β2 were purchased from R&D Systems. Buffers and salts were purchased from Merck Biosciences (Darmstadt).