Liquid dab substrate chromogen system horseradish peroxidase

Manufactured by Agilent Technologies

The Liquid DAB+ substrate chromogen system-horseradish peroxidase is a reagent used in immunohistochemistry (IHC) and other related techniques. It provides a colored substrate reaction when combined with horseradish peroxidase (HRP) enzyme labels, resulting in a brown precipitate at the site of the target antigen.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Monoclonal antibody against β actin, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (2 mentions) Glycine, by Merck Group (2 mentions) Ici 182 780, by Merck Group (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Bromodeoxyuridine (brdu), by BD (1 mentions)

Close spelling variants of this product

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2 protocols using liquid dab substrate chromogen system horseradish peroxidase

Immunocytochemical Analysis of Molecular Markers

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ICI 182,780 and E2 were obtained from Sigma (Madison, WI). Polyclonal antibodies against F4/80 and integrin-α5β1 were obtained from Abcam. Anti-BrdU monoclonol antibody came from BD Biosciences (San Jose, CA). The liquid DAB+ substrate chromogen system-horseradish peroxidase used for immunocytochemistry was obtained from Dako Cytomation (Carpinteria, CA). Penicillin, streptomycin and fetal bovine serum (FBS) were obtained from Invitrogen (Carlsbad, CA). Tris, glycine, NaCl, SDS, bovine serum albumin, and monoclonal antibody against β-actin were obtained from Sigma (Madison, WI).

Shang Z., Li Y., Hsu I., Zhang M., Tian J., Wen S., Han R., Messing E.M., Chang C., Niu Y, & Yeh S. (2016). Targeting estrogen/estrogen receptor alpha enhances Bacillus Calmette-Guérin efficacy in bladder cancer. Oncotarget, 7(19), 27325-27335.

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Immunocytochemistry Assay for Cell Signaling

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The compound ASC-J9^® was a gift from AndroScience Corp. BCG, a lyophilized preparation containing 10.5±8.7×10⁸ colony forming units (CFU), was re-suspended in PBS. HF was obtained from Sigma. Polyclonal antibodies against F4/80 and integrin-α5β1 were from Abcam, and BrdU was from BD. The liquid DAB+ substrate chromogen system-horseradish peroxidase used for immunocytochemistry was obtained from Dako Cytomation. Penicillin, streptomycin, MEM, and fetal bovine serum (FBS) were obtained from Invitrogen. Tris, glycine, NaCl, SDS, bovine serum albumin, and monoclonal antibody against β-actin were obtained from Sigma.

Shang Z., Li Y., Zhang M., Tian J., Han R., Shyr C.R., Messing E., Yeh S., Niu Y, & Chang C. (2015). Anti-androgen therapy with Hydroxyflutamide or androgen receptor degradation enhancer ASC-J9® enhances BCG efficacy to better suppress bladder cancer progression. Molecular cancer therapeutics, 14(11), 2586-2594.

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