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Dy647

Manufactured by Tecan
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Sourced in Switzerland

DY647 is a fluorescent dye that can be used for labeling and detection in various biological applications. It has an excitation maximum at 649 nm and an emission maximum at 667 nm. The dye is compatible with commonly used fluorescence detection systems.

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Lab products found in correlation

Infinite f500 reader, by Tecan (2 mentions) Cgp54626 dy647, by PerkinElmer (2 mentions) Tag lite buffer, by Tecan (2 mentions) Tag lite buffer, by PerkinElmer (2 mentions)

2 protocols using dy647

1

GABA Binding Assay in HEK293 Cells

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HEK293 cells were co-transfected with pRK5 plasmids encoding wildtype or mutant GB1 and GB2 in 96 well plates. Intact cells were washed once with Tag-lite buffer (Cisbio, France) 48 h later and incubated with both 5 nM CGP54626-DY647 (Cisbio, France) and increasing concentrations of GABA for 3 h at 4 °C in Tag-lite buffer. After three washes with Tag-lite buffer, fluorescence was measured as the specific DY647 emission spectrum (665 nm) with an Infinite F500 reader (Tecan, Switzerland).
Shaye H., Ishchenko A., Lam J.H., Han G.W., Xue L., Rondard P., Pin J.P., Katritch V., Gati C, & Cherezov V. (2020). Structural basis of the activation of a metabotropic GABA receptor. Nature, 584(7820), 298-303.
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2

GABA Binding Assay in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 cells were co-transfected with pRK5 plasmids encoding wildtype or mutant GB1 and GB2 in 96 well plates. Intact cells were washed once with Tag-lite buffer (Cisbio, France) 48 h later and incubated with both 5 nM CGP54626-DY647 (Cisbio, France) and increasing concentrations of GABA for 3 h at 4 °C in Tag-lite buffer. After three washes with Tag-lite buffer, fluorescence was measured as the specific DY647 emission spectrum (665 nm) with an Infinite F500 reader (Tecan, Switzerland).
Shaye H., Ishchenko A., Lam J.H., Han G.W., Xue L., Rondard P., Pin J.P., Katritch V., Gati C, & Cherezov V. (2020). Structural basis of the activation of a metabotropic GABA receptor. Nature, 584(7820), 298-303.
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