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6 protocols using glycerol

1

Enzymatic Assay of Phospholipase A2

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Adenosine 5′-triphosphate disodium salt hydrate ATP (99%), ammonium molybdate tetrahydrate (99%), dimethyl sulfoxide DMSO (99.9%), ethylenediaminetetraacetic acid disodium salt dehydrate EDTA (99%), potassium phosphate monobasic KH2PO4, and Trizma base (99%) were purchased from Sigma-Aldrich (St Louis, MO, USA). L-Ascorbic acid (99%) was provided by HiMedia (Mumbai, India). Magnesium chloride MgCl2 was furnished by Acros organics (New Jersey, NJ, USA). Hydrochloric acid HCl and sulfuric acid H2SO4 (96.3%) were both supplied by VWR chemicals. Glycerol was purchased from Loba Chemie (Mumbai, India). The natural molecules quercetin (95%) and thymoquinone (99%) were purchased from Sigma-Aldrich (St Louis, MO, USA). PLA2 and melittin from the Apis mellifera bee were purchased from Latoxan Laboratory (France).
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2

Chitosan-Xanthan Gum Nanocomposite Synthesis

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Chitosan from shrimp shells, 75% deacetylated,
was bought from Loba Chemie Pvt. Ltd., India. Xanthan gum pure (Food
grade), glacial acetic acid (99.5% extra pure), and glycerol (purified)
were also procured from Loba Chemie Pvt. Ltd., India. Zinc oxide nanopowder
was purchased from Sigma-Aldrich (<100 nm particle size). Anhydrous
CaCl2 and NaCl were obtained from Loba Chemie Pvt. Ltd.,
India. High-glucose Dulbecco’s modified Eagle medium (DMEM),
fetal bovine serum, MTT reagent, and Dulbecco’s phosphate buffered
saline (D-PBS) were purchased from Himedia, and dimethyl sulfoxide
(DMSO) was procured from Sigma.
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3

Vardenafil Hydrochloride Trihydrate Formulation

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Vardenafil HCl trihydrate (Batch number UT2210202) was given by El-Obour Modern Pharmaceutical Industries Co., Kaliobeya, Egypt. Sodium alginate (low viscosity grade) and glycerol were supplied by Loba Chemie Ltd., Mumbai, India. Polyvinyl pyrrolidone K30 and methylcellulose were purchased from Sigma Aldrich (St. Louis, MO, USA). Polyvinyl alcohol (molecular weight: 14,000) was purchased from BDH Chemicals Ltd., Poole, England. Sorbitol was supplied by Modernist Pantry, Eliot, ME, USA; acesulfame potassium was obtained from Sanofi Ingredients, Shanghai, China; sucralose was supplied by Asin Co., Mirkow, Poland.
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4

Extraction and Characterization of Montivipera bornmuelleri Venom

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Extraction of the venom from Montivipera bornmuelleri snake was carried out by Dr. Riyad Sadek (American University of Beirut). The venom sample was stored at -20 • C until use.
Adenosine 5 -triphosphate disodium salt hydrate ATP (99%), ammonium molybdate tetrahydrate (99%), dimethyl sulfoxide DMSO (99%), ethylenediaminetetraacetic acid disodium salt dehydrate EDTA (99%), potassium phosphate monobasic KH 2 PO 4 , Trizma base (99%) and quercetin (95%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). L-Ascorbic acid (99%) was provided by HiMedia (Mumbai, India) and Glycerol was obtained from Loba Chemie (Mumbai, India). Magnesium chloride MgCl 2 was supplied by Acros organics (Trenton, NJ, USA). Hydrochloric acid HCl and sulfuric acid H 2 SO 4 (96.3%) were provided by VWR chemicals.
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5

Extraction and Purification of Naringin

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Naringin
(≥95%)
was procured from Sigma-Aldrich (Life Science) Ltd. Choline chloride,
glycerol, and urea were acquired from Loba Chemie Ltd., Mumbai. l-glutamine was bought from Research Lab Ltd., Mumbai. All other
chemicals utilized in this study were of analytical grade and procured
from Himedia Ltd., Mumbai.
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6

Isolation and Characterization of Potato Starch

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Potato starch was isolated from fresh potato tubers variety Diacol Capiro according to Doporto et al. [14 (link)]. Briefly, potatoes were washed, sanitized (250 ppm of chlorine, 10 min), peeled and pulped with a grater. The grated potatoes were blended with water (2 L water/kg) and stored at 4 °C for 24 h. The blend was filtered using a cheesecloth, and the starch slurry was decanted at 4 °C. The supernatant was discarded, and the starch cake was recovered, dried at 40 °C for 14 h in a hot-air oven, and milled. Figure S1 shows an image of the starch granules obtained by scanning electron microscopy. Besides, some characteristics of the isolated starch are shown in Table S1.
Carvacrol (98% purity) and sodium hydroxide were purchased from Sigma Aldrich (Sigma Aldrich Inc., St. Louis, MO, USA). Glycerol was purchased from J. T. Baker (J. T. Baker Inc., New Jersey, NJ, USA) and Polysorbate 80 was purchased from Loba Chemie (Loba Chemie Inc., Mumbai, India). All other chemicals used were also of analytical grade.
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