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Pam3csk

Manufactured by Merck Group
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Pam3CSK is a synthetic triacylated lipoprotein that acts as a Toll-like receptor 2 (TLR2) agonist. It is commonly used as a research tool to study TLR2-mediated signaling and immune responses.

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Lab products found in correlation

Recombinant il 23, by R&D Systems (2 mentions) Human il 17a elisa kit, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Golgiplug, by BD (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Merck Group (1 mentions)

2 protocols using pam3csk

1

IL-17A Production Assay from Dermal Cells

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0.2 × 105 cells/100 μl dermal from HD or leprosy patients were incubated in RPMI-1640 (Invitrogen), 2 mg/ml recombinant IL-23 (R&D system, Minneapolis, MN), 1 mg/ml Pam3CSK (Merck, Rahway, NJ), 100 ng/ml LPS (Merck) or 50 mg/ml Curdlan (Merck) was added at a concentration of 2 mg/ml for 24h, the supernatant was harvest for IL-17A measurement by IL-17A Human ELISA kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions, then stimulated with presence of GolgiPlug (BD Bioscience) for 4h.
Liu Y., Shi C., Ma S., Ma Y., Lu X., Zhu J, & Yang D. (2022). The protective role of tissue-resident interleukin 17A–producing gamma delta T cells in Mycobacterium leprae infection. Frontiers in Immunology, 13, 961405.
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2

Dermal Cells Proliferation Assay

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Dermal cells suspensions were labeled with CFSE (Sigma-Aldrich) and then stimulated with 1 mg/ml of Pam3CSK (Merck) or 2 of mg/ml recombinant IL-23 (R&D system) for 4 days. Cells were harvested and stained with CD3 and TCRγδ. Proliferation was measured by expression of CFSE (Sigma-Aldrich).
Liu Y., Shi C., Ma S., Ma Y., Lu X., Zhu J, & Yang D. (2022). The protective role of tissue-resident interleukin 17A–producing gamma delta T cells in Mycobacterium leprae infection. Frontiers in Immunology, 13, 961405.
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