The largest database of trusted experimental protocols

2 protocols using monoclonal antibody against gapdh

1

Paclitaxel Resistance Mechanism Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
[3H]-Paclitaxel (37.9 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA). Dulbecco modified Eagle medium (DMEM), fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). The polyclonal antibody PA5-23652 for ABCC10 was obtained from Thermo scientific (Rockford, IL). Monoclonal antibody 3D7G8 against EphB4 was obtained from Life technologies, Invitrogen (New York, NY). NVP-BHG712 was a gift from Novartis (Basel, Switzerland). Cepharanthine was generously donated by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, colchicine, mitoxantrone, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).
+ Open protocol
+ Expand
2

Paclitaxel and Associated Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
[3H]-Paclitaxel (25.7 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA) Dulbecco modified Eagle medium (DMEM), Iscove’s DMEM, fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). Antibody D-19 against ABCC10 was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Monoclonal antibody Ab81 against c-Kit and monoclonal antibody D12E12 against phospho-c-Kit (p-c-Kit) were obtained from Cell Signaling Technologies (Beverly, MA). Masitinib was a gift from AB Science (Paris, France). Cepharanthine was generously given by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). Boron-dipyrromethene (BODIPY) FL (fluorescent) Paclitaxel was purchased from Life technologies, Invitrogen (New York, NY). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!