The AMPK activator A769662 and AMPK inhibitor Compound C were purchased from Sigma-Aldrich. Circulating leptin levels were determined using Leptin Human ELISA Kit from Invitrogen. Human recombinant leptin protein was from Thermo Fisher Scientific. Human LepR expression vector and the control were from OriGene, while human AMPKα shRNA and the control were from Santa Cruz Biotechnology. Human CD4 T cells were transfected with electroporation using Nucleofector kits from Lonza. All reagents were used according to the manufacturers’ instructions.
Human ampkα shrna
Manufactured by Santa Cruz Biotechnology
The human AMPKα shRNA is a short hairpin RNA that targets the alpha subunit of the AMP-activated protein kinase (AMPK) in human cells. AMPK is a cellular energy sensor that plays a crucial role in regulating metabolism and cellular homeostasis. The human AMPKα shRNA can be used to knockdown or silence the expression of the AMPK alpha subunit in human cell lines, allowing for the study of its function in various cellular processes.
Automatically generated - may contain errors
2 protocols using human ampkα shrna
1
AMPK Activation and Leptin Signaling
2
Molecular Modulation of T Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Notch1 inhibitor DAPT, AMPK activator A769662 and AMPK inhibitor Compound C were purchased from Sigma-Aldrich. Human AMPKα shR-NA, Notch1 shRNA, and the controls were from Santa Cruz Biotechnology. T cell transfections were performed with Nucleofector kits from Lonza. All reagents were used according to the manufacturer's instructions.
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