Annexin 5 fitc apoptosis detection kit c1062l

Propidium iodide solution (PI; B316099) was purchased from BioLegend (CA, USA). Lymphocyte Separation Medium (P8620) and TriQuick Reagent (R1100) were purchased from Solarbio (Beijing, China). The EasySep™ Mouse CD8+ T Cell Isolation Kit (19753) and EasySep™ Magnet (18000) were purchased from StemCell Technologies (Vancouver, Canada). The Annexin V-FITC Apoptosis Detection Kit (C1062L) and Red Blood Cell Lysis Buffer (C3702) were purchased from Beyotime (Shanghai, China). Pitstop-2 (ab120687) was purchased from Abcam (Illinois, USA). Hydroxy-Dynasore (HY-13863) was purchased from MedChemExpress (New Jersey, USA). CellTiter-Glo^® Luminescent Cell Viability Assay (G7570) was purchased from Promega (WI, USA). PerfectStartTM Green qPCR SuperMix (AQ601) and TransScript^® one-step gDNA removal and cDNA synthesis Supermix (at311) were purchased from TransGen Biotech (Beijing, China). The transfection reagent jetPRIME (25Y1801N5) and jetPRIME buffer (B210217) were purchased from Polyplus Transfection (Illkirch Graffenstaden, France).

Compound 6j was synthesized as reported previously [30 (link)]. Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (HY-16658B), 3-Methyladenine (3-MA, HY-19312), Necrostatin-1 (Nec-1, HY-15760), and odium 4-phenylbutyrate (4-PBA, HY-A0281) were purchased from MedChem Express (New Jersey, USA). The Cell Counting Kit-8 (CCK-8, C0038), lipid peroxidation malondialdehyde (MDA, S0131M) assay kit, and Annexin V-FITC Apoptosis detection kit (C1062L) were obtained from Beyotime (Shanghai, China). Fe²⁺ (ADS-F-QT027) and ROS detection kits (ADS-W-G010) were purchased from Aidisheng (Jiangsu, China). The 2x SYBR Green real-time qPCR Kit (AG11701) was acquired from Agbio (Jiangsu, China). The small interfering RNA of ATF3 was purchased from GenePharma (Shanghai, China). The antibody against ATF3 (A1852) and Actin (4970S) were obtained from Abclonal(Wuhan, China) and Cell signaling technology(Boston, USA), respectively.