Panc 1

The human PCa cell lines Panc-1 and AsPC-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Panc-1 was cultured in DMEM (GE Healthcare, Chicago, IL, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone, Logan, UT, USA) and antibiotics (100 U/mL of penicillin and 100 μg/mL of streptomycin; Welgene, Kyungsan, Korea). AsPC-1 was grown in RPMI1640 (GIBCO, Grand Island, NY, USA); this RPMI formulation has been modified by ATCC to include 10% FBS and 1% penicillin/streptomycin. For the experiments, these cell culture media were supplemented with 4 μg/mL of alloferon and added to cells for 3 weeks. Control cells were not treated with alloferon. The cell lines were incubated at 37 °C in a humidified incubator containing 5% CO₂.

The human embryonic kidney cell line 293T and the U87MG human glioma cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). 293FT and Lenti-X 293T cells were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Takara Bio Inc. (Shiga, Japan), respectively. The A549 human non-small cell lung cancer cell line, PANC-1 human pancreatic cancer cell line, NCI-H1299 human metastatic lung cancer cell line, Huh7 human hepatocellular carcinoma cell line, and CSC2 human glioma stem cell line were obtained from various researchers as described previously^{17 (link)–21 (link)}. The 293T, 293FT, Lenti-X 293T, U87MG, PANC-1, and Huh7 cells were cultured in Dulbecco’s modified Eagle medium (DMEM; HyClone - GE Healthcare, Chicago, IL, USA). The A549 and NCI-H1299 cells were cultured in RPMI 1640 medium (HyClone). The CSC2 glioma stem cells were cultured as described previously^{20 (link)}. All media except that used to culture CSC2 were supplemented with 10% fetal bovine serum (HyClone), 1% penicillin/streptomycin (Welgene, Gyeongsan, Republic of Korea), and 10 μg/ml ciprofloxacin (Santa Cruz Biotech, Santa Cruz, CA, USA).