The SGA drug quetiapine fumarate (cat# PHR1856) was manufactured by Sigma-Aldrich (St. Louis, MO, USA). The following antibiotics were purchased from Sigma-Aldrich: ceftriaxone disodium salt hemi(heptahydrate) (cat# C5793), levofloxacin (cat# 28266), and colistin sulfate (cat# PHR1605). Ampicillin sodium (cat# A-301-5) was purchased from Gold Biotechnology (St. Louis, MO, USA).
Ampicillin sodium
Manufactured by GoldBio
Sourced in United States
Ampicillin sodium is a powdered antibiotic compound. It is a sterile, white to off-white crystalline powder that is freely soluble in water and is commonly used in microbiology and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Levofloxacin, by Merck Group (2 mentions)
Ceftriaxone disodium salt hemi heptahydrate, by Merck Group (2 mentions)
Quetiapine fumarate, by Merck Group (2 mentions)
Colistin sulfate, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Resazurin sodium salt, by Thermo Fisher Scientific (1 mentions)
Ciprofloxacin hcl, by GoldBio (1 mentions)
Rifampicin, by GoldBio (1 mentions)
Kanamycin sulfate, by IBI Scientific (1 mentions)
Bw25113, by Horizon Discovery (1 mentions)
Hoechst 33342, by AnaSpec (1 mentions)
Metronidazole, by MP Biomedicals (1 mentions)
Tetracycline hydrochloride, by Merck Group (1 mentions)
4 protocols using ampicillin sodium
1
Antimicrobial Agents and Quetiapine
2
Antibacterial Effects of Antidepressants
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E. coli strains were purchased from the American Type Culture Collection (ATCC25922) or Dharmacon (BW25113). Typically, strains cultured in liquid yeast peptone dextrose (YPD) media (1% yeast extract, 2% peptone, and 2% glucose) or YPD plates (2% agar) at 37°C. YPD was chosen as the media for our studies as we found that the ~MIC90 of antidepressants were lower compared with Luria Broth (LB) and Tryptic Soy Broth (TSB) without changing the MICs of antibiotics (Fig. S1A ). All antidepressant and antibiotic solutions were prepared from powder stocks. All antidepressants were purchased from TCI: fluoxetine hydrochloride, sertraline hydrochloride, amitriptyline hydrochloride, nortriptyline hydrochloride, clomipramine hydrochloride. The following antibiotics were purchased from the indicated companies: ampicillin sodium (GoldBio), chloramphenicol (Sigma-Aldrich), kanamycin sulfate (IBI Scientific), rifampicin (GoldBio), and ciprofloxacin HCl (GoldBio). All were dissolved in appropriate solvent (water or ethanol) to make stocks that were then diluted into media to make agar plates or liquid cultures. Other chemicals included resazurin sodium salt (Alfa Aesar) and Hoechst 33342 (Ana Spec).
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3
Antibiotic and High-Fat Diet Modulation of Colorectal Cancer
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After one week of recovery from the last cycle of DSS, mice were randomly assigned into water (H 2 0 control group) or antibiotic (ABX) treatment group. Mice of ABX group received a three-antibiotic cocktail in the drinking water containing vancomycin hydrochloride (V-200-25, final concentration 0.5g/L, GoldBio), neomycin sulfate (# N-620-100, final concentration 1g/L, GoldBio) and ampicillin sodium (A-301-100, final concentration 1g/L, GoldBio) as previously reported (Rakoff-Nahoum et al., 2004) (link). Since it is known that mice generally keep off drinking the antibiotic cocktail for the bad taste of metronidazole, we gavaged mice with metronidazole (2 mg/mouse in 200 ul, MPBiomedicals) every other day. Fresh antibiotic cocktail was replaced every four days. After one week of ABX treatment, mice were intrasplenically injected with MC38 pLUC cells. Multiple experiments were performed simultaneously with a single control group (AOM/DSS). The same ABX mix was used to treat Apc Min/+ C3arKO, C3arKO and WT mice for one week. High fat diet experiment For the High Fat Diet (HFD) experiment, eight weeks old C57BL/6J male mice were maintained on chow diet as control diet or 60% lard high-fat diet (HFD, TD.06414) for one week. The HFD TD.06414 was purchased from Envigo. At the experimental end points, mice were sacrificed for organ harvest.
4
Antibiotics and Antipsychotic Drug Procurement
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The second-generation antipsychotic drug quetiapine fumarate (catalog #PHR1856) was manufactured by Sigma-Aldrich (St. Louis, MO).
The following antibiotics were purchased from Sigma-Aldrich: amikacin disulfate (catalog #A1774), ceftriaxone disodium salt hemi(heptahydrate) (#C5793), levofloxacin (#28266), and tetracycline hydrochloride (#T7660). Ampicillin sodium (cat #A-301-5) was purchased from Gold Biotechnology (St. Louis, MO).
The following antibiotics were purchased from Sigma-Aldrich: amikacin disulfate (catalog #A1774), ceftriaxone disodium salt hemi(heptahydrate) (#C5793), levofloxacin (#28266), and tetracycline hydrochloride (#T7660). Ampicillin sodium (cat #A-301-5) was purchased from Gold Biotechnology (St. Louis, MO).
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