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Mab098

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MAB098 is a mouse monoclonal antibody. It is designed for detection applications.

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Lab products found in correlation

Mang20, by Abcam (2 mentions) Bio plex amine coupling kit, by Bio-Rad (1 mentions)

3 protocols using mab098

1

Multiplex Luminex Assay for Angiogenic Factors

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Vitreous samples of patients with wAMD, diabetic retinopathy, retinal vein occlusion, and macular hole were collected by Dr. M. Koss, University of Heidelberg (Koss et al, 2011). Collection of samples was performed after local full ethics committee approval (57/08) in accordance with the European Guidelines for Good Clinical Practice and the Declaration of Helsinki. Informed consent was obtained from each patient before the start of therapy. Samples were stored at −70°C and transferred to Roche for the analysis of ANG‐1 and ANG‐2 using a multiplex Luminex kit produced in‐house using the following reagents: anti‐ANG‐1 capture (R&D, MAB9231) and anti‐ANG‐1 detection (Novus Biologicals, NB110‐85464), anti‐ANG‐2 capture (R&D, MAB098) and anti‐ANG‐2 detection (R&D, BAM0981). Capture antibodies were coupled to Luminex beads using the Bio‐Plex Amine Coupling Kit (Bio‐Rad 171406001). Vitreous samples diluted one‐third were incubated with capture antibody bead for 2 h. After washing the beads, biotinylated detector antibodies were added and incubated with the beads for 1 h. Streptavidin‐conjugated fluorescent protein, R‐phycoerythrin (BD, 554061), was then added and incubated for 30 min. After washing, the beads were analyzed using a Luminex 100 detection system.
Regula J.T., Lundh von Leithner P., Foxton R., Barathi V.A., Cheung C.M., Bo Tun S.B., Wey Y.S., Iwata D., Dostalek M., Moelleken J., Stubenrauch K.G., Nogoceke E., Widmer G., Strassburger P., Koss M.J., Klein C., Shima D.T, & Hartmann G. (2016). Targeting key angiogenic pathways with a bispecific CrossMAb optimized for neovascular eye diseases. EMBO Molecular Medicine, 8(11), 1265-1288.
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2

ANGPT2 Measurement in EC and Tissues

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For measurement of ANGPT2 in human EC supernatant, the cells were treated and harvested at indicated times. ELISA was performed with standard sandwich ELISA assay using anti-ANGPT2 antibodies from R&D, recombinant human ANGPT2 (625-AN-025), human ANGPT2 Mab (MAB098), human ANGPT2 biotinylated Mab (Bam0981). For measurement of ANGPT2 in mouse cells and tissues, the Angpt2 mouse ELISA kits were from R&D (MANG20) and ABCAM (ab171335). For Western blotting, mouse tissues were snap-frozen and tissues were homogenized in RIPA lysis buffer prior to SDS-PAGE. For ELISA assay, fresh mouse tissues were minced followed by incubation in serum-free medium for 2 h. ANGPT2 proteins in Supernatants were measured.
Jenny Zhou H., Qin L., Zhang H., Tang W., Ji W., He Y., Liang X., Wang Z., Yuan Q., Vortmeyer A., Toomre D., Fuh G., Yan M., Kluger M.S., Wu D, & Min W. (2016). Augmented endothelial exocytosis of angiopoietin-2 resulting from CCM3-deficiency contributes to the progression of cerebral cavernous malformation. Nature medicine, 22(9), 1033-1042.
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3

ANGPT2 Measurement in EC and Tissues

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For measurement of ANGPT2 in human EC supernatant, the cells were treated and harvested at indicated times. ELISA was performed with standard sandwich ELISA assay using anti-ANGPT2 antibodies from R&D, recombinant human ANGPT2 (625-AN-025), human ANGPT2 Mab (MAB098), human ANGPT2 biotinylated Mab (Bam0981). For measurement of ANGPT2 in mouse cells and tissues, the Angpt2 mouse ELISA kits were from R&D (MANG20) and ABCAM (ab171335). For Western blotting, mouse tissues were snap-frozen and tissues were homogenized in RIPA lysis buffer prior to SDS-PAGE. For ELISA assay, fresh mouse tissues were minced followed by incubation in serum-free medium for 2 h. ANGPT2 proteins in Supernatants were measured.
Jenny Zhou H., Qin L., Zhang H., Tang W., Ji W., He Y., Liang X., Wang Z., Yuan Q., Vortmeyer A., Toomre D., Fuh G., Yan M., Kluger M.S., Wu D, & Min W. (2016). Augmented endothelial exocytosis of angiopoietin-2 resulting from CCM3-deficiency contributes to the progression of cerebral cavernous malformation. Nature medicine, 22(9), 1033-1042.
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