Alexa fluor 594 donkey anti rabbit igg h l antibody

Tris-base, Gly, NaCl, SDS, MTT, and BSA were purchased from Sigma-Aldrich (St. Louis, MO, USA). The LightShift^® Chemiluminescent EMSA Kit was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Anti-phospho-IKKα/β, anti-IKKβ, anti-IκBα anti-IKKα, anti-phospho-IκBα antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-phospho-p65, anti-p65, anti-survivin, anti-Bcl-2, anti-Bcl-xl, anti-COX-2, anti-VEGF, anti-MMP-9, anti-PARP, anti-Lamin B, and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Alexa Fluor^® 594 donkey anti-rabbit IgG (H + L) antibody was obtained from Life Technologies (Grand Island, NY, USA). The FITC Annexin V Apoptosis Detection Kit I was purchased from (BD Biosciences, Becton-Dickinson, Franklin Lakes, NJ, USA).

Fangchinoline (FCN, Figure 1A) was purchased from Chem faces (Wuhan, Hubei, China). FCN was stored in 100 mM stock solution with dimethyl sulfoxide at −20 °C and diluted in cultured media for in vitro experiments. LightShift^® Chemiluminescent EMSA kit was purchased from Thermo Fisher Scientific Inc. Alexa Fluor^® 594 donkey anti-rabbit IgG (H + L) antibody was obtained from Life Technologies (Grand Island, NY, USA). Z-DEVD-FMK (caspase-3 inhibitor) was purchased from CALBIOCHEM (San Diego, CA, USA).