Materials were obtained from the following suppliers: female rat non-cancerous type-II alveolar cells (CC-149, L2 cells) and fetal bovine serum (FBS), ATCC; low glucose DMEM, Hyclone. Phenol red-free low glucose DMEM, 17β-estradiol (E2) and 3,3′, 5-triiodo-L-thyronine sodium salt (T3), Sigma-Aldrich; charcoal-stripped FBS and 100X antibiotic-antimycotic, Invitrogen; Trypsin-EDTA .05%, VWR; Na-pyruvate, Cellgro; Hanks buffered saline solution (HBSS), Lonza; MTT, PGE2, and Apotox-Glo Triplex assay kits from Roche, Cayman Chemical, and Promega, respectively. Promega generously provided GSH/GSSG-Glo assay prior to public release.
Phenol red free low glucose dmem
Manufactured by Merck Group
Phenol red-free low glucose DMEM is a cell culture medium formulation from Merck Group. It is a basal medium designed to support the growth and maintenance of various cell types in vitro. The medium is devoid of the pH indicator phenol red and contains a reduced concentration of glucose compared to standard DMEM formulations.
Automatically generated - may contain errors
Lab products found in correlation
Napyruvate, by Corning (1 mentions)
Hank s buffered saline solution hbss, by Lonza (1 mentions)
Gsh gssg glo assay, by Promega (1 mentions)
Dichloroacetate, by Merck Group (1 mentions)
Trypsin edta 0.05, by Avantor (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
2 protocols using phenol red free low glucose dmem
1
Rat Alveolar Cell Culture Assay
2
Fetal Bovine Serum Acquisition and Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville GA). Phenol-red-free, low glucose DMEM, and dichloroacetate (DCA) were purchased from Sigma-Aldrich (St. Louis, MO). 100x antibiotic-antimycotic was purchased from Gibco (Carlsbad, CA). Trypsin-EDTA 0.05% was obtained from VWR (Radnor, PA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!