Q exactive gc orbitrap hrms detector

A Trace 1310 GC system equipped with a TraceGOLD TG-5MS column (30 m, 0.25 mm, 0.25 µm), coupled to a Q-Exactive GC Orbitrap HRMS detector, all from Thermo Fisher Scientific (Waltham, MA, USA), were used for GC-HRMS analysis.
A Conterm laboratory oven, from J.P. Selecta (Barcelona, Spain); an Advanced IR vortex mixer, from VELP Scientifica (Usmate Velate, Italy); and an Allegra X-15R centrifuge, from Beckman Coulter (Indianapolis, IN, USA), were also used.

A Trace 1310 GC system equipped with a TraceGOLD TG-5MS column (30 m, 0.25 mm, 0.25 µm), coupled to a Q-Exactive GC Orbitrap HRMS detector, all from Thermo Fisher Scientific (Waltham, MA, USA), were used. The injection volume was 1 µL (splitless mode) and the inlet was set at 280 °C. The GC operated in constant flow mode at 1.2 mL min⁻¹ of helium as carrier gas, using the following oven temperature program: 40 °C, held for 5 min; 5 °C min⁻¹ up to 315 °C, held for 10 min. The MS transfer line was set at 300 °C. The EI ion source operated at 70 eV, and the ion source temperature was set at 250 °C. The acquisition was performed in full scan mode with a resolving power of 60,000 FWHM and a mass range from 40 to 500 m/z.
To perform retention index (RI) calculation, standard n-alkane mixtures, C8-C20 and C10-C40 (all even), were injected with the same conditions.