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Spin x tube filters tubes

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Spin-X tube filters are laboratory centrifuge tubes designed for rapid and efficient separation of particles and liquid components. The tubes feature a built-in membrane filter that retains particles while allowing the liquid to pass through during centrifugation. This core function facilitates sample clarification, purification, and preparation for various analytical and experimental procedures.

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Lab products found in correlation

Precellys homogenizer, by Bertin Technologies (2 mentions) Bsa fraction 5, by Roche (2 mentions) Zirconia beads, by Bertin Technologies (2 mentions) Middlebrook 7h11 agar, by BD (2 mentions)

2 protocols using spin x tube filters tubes

1

Metabolomic Analysis of Mycobacterial Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metabolomics experiments and analysis were performed according to published literature51 (link). One milliliter of mid-log phase cultures (0.8 −1 OD580) was passed through 0.22-μm nylon filters and allowed to grow at 37°C for 5 days on Middlebrook 7H11 agar (BD) supplemented with 0.2% glycerol, 0.85g/L NaCl, 2 g/L D-glucose, and 5 g/L Fraction V BSA (Roche). On Day 6, Mtb -laden filters were transferred onto a reservoir containing 7H9 media (without tyloxapol) with 50 ng/ml ATc and left at 37°C for 24 hrs. Next, these acclimatized filters were exposed to fresh 7H9 media with and without INH (7.2 μM) and ATc. After 24 hours, these filters were quenched in precooled (−40°C) mix of acetonitrile/methanol/water (40%:40%:20%). Metabolites were extracted by bead beating using 0.1 mm Zirconia beads and Precellys homogenizer (Bertin Instruments, Rockville, MD). Lysates were centrifuged and decontaminated by passing through Spin-X tube filters tubes (0.22 μm, Sigma).
Ma S., Morrison R., Hobbs S.J., Soni V., Farrow-Johnson J., Frando A., Fleck N., Grundner C., Rhee K.Y., Rustad T.R, & Sherman D.R. (2020). Transcriptional Regulator Induced Phenotype screen reveals drug potentiators in Mycobacterium tuberculosis. Nature microbiology, 6(1), 44-50.
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2

Metabolomic Analysis of Mycobacterial Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metabolomics experiments and analysis were performed according to published literature51 (link). One milliliter of mid-log phase cultures (0.8 −1 OD580) was passed through 0.22-μm nylon filters and allowed to grow at 37°C for 5 days on Middlebrook 7H11 agar (BD) supplemented with 0.2% glycerol, 0.85g/L NaCl, 2 g/L D-glucose, and 5 g/L Fraction V BSA (Roche). On Day 6, Mtb -laden filters were transferred onto a reservoir containing 7H9 media (without tyloxapol) with 50 ng/ml ATc and left at 37°C for 24 hrs. Next, these acclimatized filters were exposed to fresh 7H9 media with and without INH (7.2 μM) and ATc. After 24 hours, these filters were quenched in precooled (−40°C) mix of acetonitrile/methanol/water (40%:40%:20%). Metabolites were extracted by bead beating using 0.1 mm Zirconia beads and Precellys homogenizer (Bertin Instruments, Rockville, MD). Lysates were centrifuged and decontaminated by passing through Spin-X tube filters tubes (0.22 μm, Sigma).
Ma S., Morrison R., Hobbs S.J., Soni V., Farrow-Johnson J., Frando A., Fleck N., Grundner C., Rhee K.Y., Rustad T.R, & Sherman D.R. (2020). Transcriptional Regulator Induced Phenotype screen reveals drug potentiators in Mycobacterium tuberculosis. Nature microbiology, 6(1), 44-50.
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