Human angiotensin 1

Manufactured by Merck Group

Human angiotensin I is a lab equipment product. It is a peptide hormone that plays a role in the renin-angiotensin-aldosterone system, which regulates blood pressure and fluid balance.

Automatically generated - may contain errors

Lab products found in correlation

Bradykinin, by Merck Group (3 mentions) Human angiotensin 2, by Merck Group (3 mentions) Substance p, by Merck Group (3 mentions) Neurotensin, by Merck Group (2 mentions) Porcine angiotensinogen, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Kemptide, by Merck Group (2 mentions) Optima lc ms grade, by Thermo Fisher Scientific (1 mentions) Met5 enkephalin, by Merck Group (1 mentions) Fibrinopeptide a, by Merck Group (1 mentions) Melittin, by Merck Group (1 mentions)

3 protocols using human angiotensin 1

Peptide Characterization by Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Water, methanol, and trifluoroacetic acid (TFA) used as solvents and additives throughout these experiments were obtained from Fisher Scientific (Optima LC/MS grade). The modified peptide renin substrate I (97%), human angiotensin I (90%), human angiotensin II (93%), bradykinin (98%), substance P (95%), Met⁵-enkephalin (95%), and [d-Ala²,d-Leu⁵]-enkephalin (95%) were all purchased as acetate salts from Sigma-Aldrich (St. Louis, MO). Biotinylated, phosphorylated SAMS peptide was obtained from Enzo Life Sciences (Farmingdale, NY). Support solutions for the SCGD were prepared by dilution of concentrated, trace metal grade hydrochloric acid (J.T. Baker, Center Valley, PA) with deionized water of 18.2-MΩ resistivity, prepared in-house with a mixed-bed ion-exchange deionization unit.

Schwartz A.J., Shelley J.T., Walton C.L., Williams K.L, & Hieftje G.M. (2016). Atmospheric-pressure ionization and fragmentation of peptides by solution-cathode glow discharge †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc02032a Click here for additional data file.. Chemical Science, 7(10), 6440-6449.

+ Open protocol

+ Expand

Preparation of Peptide Standards for Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

1 mg/mL stock solutions of each peptide (human angiotensin I, human angiotensin II, bradykinin, fibrinopeptide A, kemptide, neurotensin, porcine angiotensinogen, and substance P, all purchased from Sigma-Aldrich) were prepared in 0.1% formic acid (FA, Sigma-Aldrich, St. Louis, MO) in 10% acetonitrile (ACN, Fisher Scientific, Pittsburgh, PA) and deionized water. (Barnstead Nanopure Infinity System, Dubuque, IA). A peptide mixture containing 10 μM of each peptide was prepared from the stock solution in the same solvent mixture. Prior to MS analysis the peptide mixture solutions with final concentrations of 1 μM and 100 nM for each peptide were prepared by further dilution.

Cox J.T., Marginean I., Smith R.D, & Tang K. (2014). On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces. Journal of the American Society for Mass Spectrometry, 26(1), 55-62.

+ Open protocol

+ Expand

Peptide Quantification by Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The ESI solvent consisted of 0.1% formic acid (FA, Sigma-Aldrich, St. Louis, MO) in 10% acetonitrile (ACN, Fisher Scientific, Pittsburgh, PA) and deionized water (Barnstead Nanopure Infinity System, Dubuque, IA). Stock solutions of 9 peptides (human angiotensin I, human angiotensin II, bradykinin, fibrinopeptide, kemptide, melittin, neurotensin, porcine angiotensinogen, and substance P, all purchased from Sigma-Aldrich) were prepared in the ESI solvent. Aliquots from the stock solutions were mixed and diluted to a final concentration of 1 μM for each peptide.

Cox J.T., Marginean I., Kelly R.T., Smith R.D, & Tang K. (2014). Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures. Journal of the American Society for Mass Spectrometry, 25(12), 2028-2037.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!