Anti c cbl

The following antibodies were used: anti-Src (05–184; Merck, Darmstadt, Germany), anti-Yes (610375; BD Biosciences, San Jose, CA, USA), anti-Lyn (sc-7274; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Csk (610080; BD Biosciences), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (2275-PC-100; R&D Systems, Minneapolis, MN, USA), anti-p-Tyr (05–1050; Merck), anti-Src family phospho-Y418 (p-SFKs A-loop; ab40660; Abcam, Cambridge, UK), anti-p-Src Y530 (sc-166860; Santa Cruz Biotechnology), anti-p-Lyn Y508 (CSB-PA000691; Cusabio, Wuhan, China)), anti-Fyn (sc-434; Santa Cruz Biotechnology), anti-E-cadherin (#3195; Cell Signaling Technology, Danvers, MA, USA), anti-vimentin (V6630; Sigma-Aldrich), anti-Cbl-b (sc-8006; Santa Cruz Biotechnology) and anti-c-Cbl (sc-1651; Santa Cruz Biotechnology). Horseradish peroxidase-conjugated anti-mouse IgG (#7076; Cell Signaling Technology) and anti-rabbit IgG (711-035-152; Jackson Immunoresearch, West Grove, PA, USA) antibodies were used for Western blotting.

Anti-HER2 was purchased from NeoMarker (Waltham, MA, USA), anti-ER, anti-PR, anti-c-Src, anti-p-HER2 (Tyr1248), anti-p-c-Src (Tyr416), and anti-p-ER (Ser118) were purchased from CST (Danvers, MA, USA). Anti-actin, anti-c-Cbl, anti-Caveolin1 and Anti-HER2 labeled with phycoerythrin were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Tamoxifen, 17-β estradiol, Nystatin, PP2were purchased from Sigma (St. Louis, MO, USA). The c-Cbl-3 × flag-CMV9 plasmid was constructed by Taihe Biotechnology (Beijing, China). The HER2-pEGFP-N1 plasmid was constructed by GENEWIZ (Suzhou, China). The PSVL-cbl-70Z plasmid was kindly gift from Professor Kiyonao Sada (Kobe University Graduate School of Medicine). The lentiviral system was purchased from Genechem (Shanghai, China).