Pfu polymerase

The human DNMT1 gene was amplified through polymerase chain reaction (PCR) using Pfu polymerase (Biofact, PD302-50h). Primers were screened with Primer3web version 4.1.0 and synthesized by Bioneer Corporation (Korea). The amplicon and primer sequences for DNMT1 are noted in Fig. S1. The PCR products were purified with a HiGene Gel & PCR Purification system (Biofact, GP104-100), and cloned into an All in One PCR Cloning vector (Biofact, VT201-020). The cloned vector constructs were extracted using an NucleoBond Xtra Midi kit (Machery-Nagel, 740410.50).

Nonylphenol was bought from Kanto Chemical (Tokyo, Japan). Primers and the template to produce the single-stranded DNA (ssDNA) library and amplify the elution product were synthesized by Bioneer (Daejeon, Korea). Pfu polymerase was purchased from Biofact (Daejeon, Korea), and restriction endonucleases and DNA ligases were obtained from Takara Bio (Shiga, Japan). Reduced graphene oxide (rGO) was purchased from the Graphene Supermarket (Calverton, NY, USA), and bisphenol A (BPA), phenyl phenol (PP), phenol, and diethylhexyl phthalate (DEHP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Penicillin G (PG) was obtained from Duchefa Biochemie (Haarlem, Netherlands). All other chemicals were obtained from commercial sources and were the highest quality available.