from non-irradiated C57BL/6 mice, transplanted mice, and transplanted mice with
the injected BMC (30 d after conditioning). Total DNA was extracted using TRIZOL
following tissue homogenization in a Bullet Blender Storm BBX24 instrument (Next
Advance, Inc.). DNA was quantified by UV-Vis and 1 μg of DNA per sample
was used as input for real-time PCR. A standard curve of mouse sjTREC plasmid
was used to calculate the absolute number of single joint TRECS (sjTRECs) per
sample.