Anti phospho h2ax s139

Immunofluorescence assays were performed as previously reported (45 (link)). The following antibodies were used for immunofluorescence staining: anti-NONO (#611279, 1:500) from BD Transduction Laboratories; anti-SFPQ (#A301-320A, 1:200) from Bethyl Laboratories, Inc.; anti-phospho H2AX S139 (#ab11174, 1:1000) from Abcam.

Western blot and detection were performed as previously described (45 (link)). The following antibodies were used for Western blotting: anti-RNF8 (B-2) (#sc-271462, 1:5000), anti-GFP (#sc-9996, 1:3000), anti-Ub (A-5) (#sc-166553, 1:1000), anti-CDC25A (F-6) (#sc-7389, 1:1000), anti-p53 (#sc-126, 1:1000) and anti-HSP90 (H-114) (#sc-7479, 1:2000) from Santa Cruz Biotechnology; anti-phospho H2AX S139 (#ab11174, 1:20000), anti-H2AX (#ab11175, 1:10 000) and anti-TOPBP1 (#ab2402, 1:5000) from Abcam; anti-phospho CHK1 S345 (#2348, 1:2500), anti-CHK1 (#sc-8408, 1:3000) and anti-MYC (#2276, 1:2000) from Cell Signaling Technology; anti-FLAG (#F3165, 1:10 000), anti-biotin (#B7653, 1:10 000) and anti-β-actin (#A2228, 1:10000) from Sigma; anti-NONO (#611279, 1:10000) from BD Transduction Laboratories; anti-T7 (#69522, 1:10000) from Novagen; anti-HA (#clone 12CA5, 1:1000) from Roche; anti-ATRIP (#A300-095A, 1:2000), anti-XPC (#A301-122A, 1:2000) and anti-SFPQ (#A301-320A, 1:2000) from Bethyl Laboratories, Inc.; anti-K48-specific ubiquitin (#clone Apu2.07, 1:1000) and anti-K63-specific ubiquitin (#clone Apu3.A8, 1:1000) from Genentech Inc.