Hydroxybenzotriazole hydrate

Manufactured by Merck Group

Sourced in Germany

1-Hydroxybenzotriazole hydrate is a reagent commonly used in organic chemistry for the activation of carboxylic acids in various coupling reactions. It functions as an activating agent and coupling promoter. The exact chemical formula is C6H5N3O·xH2O, where x represents the number of water molecules in the hydrate.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

1-hydroxybenzotriazole hydrate (23 citations) Hydroxybenzotriazole (20 citations) 1-hydroxybenzotriazole hydrate (HOBt) (12 citations)

2 protocols using hydroxybenzotriazole hydrate

Purification and Analysis of C1-Inhibitor

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ammonium bicarbonate (AmBiC), hydroxybenzotriazole hydrate (HOBt), iodoacetamide (IAA), 2-mercaptoethanol, Nonidet P-40 (NP-40), PBS, formic acid (FA), sDHB (2-hydroxy-5-methoxy-benzoic acid and 2,5-dihydroxybenzoic acid, 1:9) as well as a 50% sodium hydroxide (NaOH) solution were obtained from Sigma-Aldrich (Steinheim, Germany). Sodium hydrogen phosphate dihydrate (Na₂HPO₄x2H₂O), sodium bicarbonate, DTT, ethanol, potassium dihydrogen phosphate, SDS, sodium chloride and TFA were purchased from Merck (Darmstadt, Germany). 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) originated from Fluorochem (Hadfield, UK) and HPLC SupraGradient ACN from Biosolve (Valkenswaard, The Netherlands).
Commercial C1-Inh (Cetor®), isolated from pooled cryo- and 4F-depleted plasma, using ion exchange chromatography and PEG precipitation, was obtained from Sanquin, Amsterdam, The Netherlands.

Stavenhagen K., Kayili H.M., Holst S., Koeleman C.A., Engel R., Wouters D., Zeerleder S., Salih B, & Wuhrer M. (2017). N- and O-glycosylation Analysis of Human C1-inhibitor Reveals Extensive Mucin-type O-Glycosylation. Molecular & Cellular Proteomics : MCP, 17(6), 1225-1238.

+ Open protocol

+ Expand

Glycoprotein Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ethanol, trifluoroacetic acid (TFA), sodium dodecyl sulfate (SDS), disodium hydrogen phosphate dihydrate (Na₂HPO₄ × 2H₂O), hydrochloric acid (HCl), and sodium chloride (NaCl) were purchased from Merck (Darmstadt, Germany). Hydroxybenzotriazole hydrate, 50% sodium hydroxide (NaOH), and Nonidet P-40 were obtained from Sigma-Aldrich. 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride originated from Fluorochem (Hadfield, UK). Peptide:N-glycosidase F was bought from Roche Diagnostics (Mannheim, Germany), 2,5-dihydroxybenzoic acid from Bruker Daltonics (Bremen, Germany), and HPLC SupraGradient ACN from Biosolve (Valkenswaard, The Netherlands). Milli-Q deionized water (R > 18.2 mΩ cm⁻¹; Millipore Q-Gard 2 system, Millipore, Amsterdam, The Netherlands) was used in this study.

Bondt A., Rombouts Y., Selman M.H., Hensbergen P.J., Reiding K.R., Hazes J.M., Dolhain R.J, & Wuhrer M. (2014). Immunoglobulin G (IgG) Fab Glycosylation Analysis Using a New Mass Spectrometric High-throughput Profiling Method Reveals Pregnancy-associated Changes. Molecular & Cellular Proteomics : MCP, 13(11), 3029-3039.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!