Immunohistochemical studies were performed on five-micrometer thick sections prepared from formalin-fixed and paraffin embedded tissue. Most of the immunohistochemistry was done at The Johns Hopkins Hospital, where the primary antibodies were: p16 (clone INK4a; MTM Laboratories, Heidelberg, Germany); p63 (clone 4A4; Cell Marque Corp., Rocklin, CA); p40 (clone Ab-1; Oncogene Research Products, Cambridge, MA), muscle specific actin (clone HHF35; Ventana; Phoenix, AZ); calponin (clone CALP; Dako, Carpinteria, CA); S100 (clone 4C4.9; Ventana); c-kit (clone YR145; Cell Marque); and AE1/AE3 (clone PCK26; Ventana). For a minority of cases, immunohistochemistry had been done at an outside institution and the reported results were recorded. As a surrogate marker for the presence of HPV, p16 immunostaining was regarded as positive only if staining was both nuclear and cytoplasmic, and present in ≥70% of the tumor cells.
S100 clone 4c4.9
Manufactured by Roche
Sourced in United States
The S100 (clone 4C4.9) is a lab equipment product manufactured by Roche. It is a tool used in scientific research and analysis. The core function of the S100 (clone 4C4.9) is to perform a specific task or measurement, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
2 protocols using s100 clone 4c4.9
1
Immunohistochemical Profiling of Tissue Samples
2
Immunohistochemistry of Tumor Markers
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Immunohistochemistry was performed by using anti VEGF, COX-2, EGFR, ProEx-C and TERT antibodies [21] [22] [23] [24] [25] . Vascular endothelial growth factor in a 1/20 dilution (a mouse monoclonal antibody (H11) Catalogue # MA5-13182, Invitrogen, Thermo Fisher Scientific, USA), COX-2 a rabbit monoclonal anti-COX-2 antibody (diluted 1: 50; BIOCARE MEDICAL, USA), EGFR in 2 µg/ml dilution (a mouse monoclonal antibody (JH121) Catalogue # MA5-13070, Invitrogen, Thermo Fisher Scientific, USA) ProExC (prediluted, clone MCM2 26H6.19, MCM2 27C5.6, TOP2A SWT3D1; TriPath Imaging Inc, Burlington, NC), TERT, anti-telomerase catalytic subunit (RABBIT) antibody-600-401-252S (Rockland Immunochemicals, Inc., Limerick, PA, USA), muscle-specific actin (clone HHF35; Ventana), calponin (clone CALP; Dako), and S100 (clone 4C4.9; Ventana [26] [27] [28] [29] [30] .
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