Recombinant mouse TNF-α (Shenandoah Biotechnology, Warwick, PA) was injected intravenously via the tail vein into WT and miR-21−/− mice in a volume of 0.2 ml (9 μg in endotoxin-free PBS); animals were observed hourly for survival. In a separate set of experiments, mice were sacrificed 3 h after TNF-α injection, and serum and tissue samples of ileum, kidney and liver were collected. Histopathology of tissue damage of ileum, kidney and liver were evaluated by a pathologist in a double-blind manner on paraffin sections on a scale ranging from 0 (completely unaffected tissue), 1 (negligible damage), 2 (minor damage), 3 (moderate damage), to 4 (severe damage).
Recombinant mouse tnf α
Manufactured by Fujifilm
Recombinant mouse TNF-α is a laboratory reagent produced by Fujifilm. It is a protein that functionally mimics the natural tumor necrosis factor alpha found in mice. This product is intended for use in research applications.
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Lab products found in correlation
2 protocols using recombinant mouse tnf α
1
Evaluating TNF-α-Induced Tissue Damage
2
Evaluating TNF-α-Induced Tissue Damage
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Recombinant mouse TNF-α (Shenandoah Biotechnology, Warwick, PA) was injected intravenously via the tail vein into WT and miR-21−/− mice in a volume of 0.2 ml (9 μg in endotoxin-free PBS); animals were observed hourly for survival. In a separate set of experiments, mice were sacrificed 3 h after TNF-α injection, and serum and tissue samples of ileum, kidney and liver were collected. Histopathology of tissue damage of ileum, kidney and liver were evaluated by a pathologist in a double-blind manner on paraffin sections on a scale ranging from 0 (completely unaffected tissue), 1 (negligible damage), 2 (minor damage), 3 (moderate damage), to 4 (severe damage).
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